Detailed information of TA system TA217926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 193087..193685
• Replicon: chromosome
• Accession: NZ_CP084007
• Organism: Flavonifractor plautii strain NBRC 113805

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LCR02_RS00915
• Protein ID: WP_055271538.1
• Coordinates: 193347..193685 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A4R2LWB8
• Locus tag: LCR02_RS00910
• Protein ID: WP_021630507.1
• Coordinates: 193087..193353 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCR02_RS00885 (LCR02_00885)	188167..188880	+	714	WP_009261353.1	hypothetical protein	-
LCR02_RS00890 (LCR02_00890)	189250..189732	+	483	WP_021631604.1	sigma-70 family RNA polymerase sigma factor	-
LCR02_RS00895 (LCR02_00895)	189930..190208	-	279	WP_009261352.1	helix-turn-helix domain-containing protein	-
LCR02_RS00900 (LCR02_00900)	190205..191956	-	1752	WP_131971895.1	type IV secretory system conjugative DNA transfer family protein	-
LCR02_RS00905 (LCR02_00905)	192086..193009	-	924	WP_055271541.1	DUF3991 and toprim domain-containing protein	-
LCR02_RS00910 (LCR02_00910)	193087..193353	+	267	WP_021630507.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LCR02_RS00915 (LCR02_00915)	193347..193685	+	339	WP_055271538.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LCR02_RS00920 (LCR02_00920)	193732..195180	-	1449	WP_131971897.1	MobP3 family relaxase	-
LCR02_RS00925 (LCR02_00925)	195177..195599	-	423	WP_131971899.1	hypothetical protein	-
LCR02_RS00930 (LCR02_00930)	195922..196737	-	816	WP_156621244.1	hypothetical protein	-
LCR02_RS00935 (LCR02_00935)	196734..198191	-	1458	WP_225123335.1	ankyrin repeat domain-containing protein	-
LCR02_RS00940 (LCR02_00940)	198178..198438	-	261	WP_021631474.1	DUF6103 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	134541..213822	79281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-101)

Antitoxin

• SpoVT-AbrB domain (6-41)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12766.54 Da
• Isoelectric Point: 5.5275

>T217926 WP_055271538.1 NZ_CP084007:193347-193685 [Flavonifractor plautii]
MVMIFEQGDIVYLDFDPQSGHEQKSRRPALVISNNLFNRVSSLTMVCPITHTDRGHPFHLRLDERTRTDGVILCDQARML
DLNSRHASFVERAPEDISAEAVDMVIGFLEPK

Nucleotide

• Length: 339 bp

>T217926 NZ_CP114894:c4192422-4192081 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9960.28 Da
• Isoelectric Point: 5.1473

>AT217926 WP_021630507.1 NZ_CP084007:193087-193353 [Flavonifractor plautii]
MYATIQKWGNSQGLRIPKALLDAVGLKEHDRVELVQMDDGINIRRASATPHRTLEERLIAFYGKPIDQIGRIEGEELDWG
KAEGSEAW

Nucleotide

• Length: 267 bp

>AT217926 NZ_CP114894:c4192760-4192443 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4R2LWB8