Detailed information of TA system TA021789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5720095..5720736
• Replicon: chromosome
• Accession: NC_008596
• Organism: Mycobacterium smegmatis str. MC2 155

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MSMEG_RS27155
• Protein ID: WP_011730672.1
• Coordinates: 5720095..5720538 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I7FKW8
• Locus tag: MSMEG_RS27160
• Protein ID: WP_011730673.1
• Coordinates: 5720548..5720736 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSMEG_RS27135 (MSMEG_5630)	5715578..5716315	+	738	WP_011730668.1	GntR family transcriptional regulator	-
MSMEG_RS27140 (MSMEG_5631)	5716317..5717849	+	1533	WP_011730669.1	cyclohexanecarboxylate-CoA ligase	-
MSMEG_RS27145 (MSMEG_5632)	5717850..5718623	+	774	WP_011730670.1	SDR family oxidoreductase	-
MSMEG_RS27150 (MSMEG_5633)	5718620..5720092	+	1473	WP_011730671.1	long-chain fatty acid--CoA ligase	-
MSMEG_RS27155 (MSMEG_5634)	5720095..5720538	-	444	WP_011730672.1	SRPBCC family protein	Toxin
MSMEG_RS27160 (MSMEG_5635)	5720548..5720736	-	189	WP_011730673.1	antitoxin	Antitoxin
MSMEG_RS27165 (MSMEG_5636)	5720762..5723137	-	2376	WP_011730674.1	cation-translocating P-type ATPase	-
MSMEG_RS27170 (MSMEG_5637)	5723151..5724758	-	1608	WP_162139558.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T21789 WP_011730672.1 NC_008596:c5720538-5720095 [Mycolicibacterium smegmatis MC2 155]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T21789 NC_008596:c5720538-5720095 [Mycolicibacterium smegmatis MC2 155]
ATGGCCAAACTTTCCGTCTCCGTCGAAGTTCCATTGCCGCCGGAGCAGGCGTGGGAGTATGCCTCCGACCTGTCCCGGTA
CCACGAGTGGCTCAGCATTCACCGGGCCTGGCGCTCGAAGCTGCCCGAGACCCTGGAGAAGGGCACGGTGATCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAGGTGGACGTTGGTCCACTACAAGCCCCCGCAGGCGCTCACACTCAAC
GGTGACGGCCGCGGCGGGGTCAAGGTCAAGCTGATCGGCAAGATCAAGCCCACCGACAACGGCGCGACGGTGGGCTTCGA
CCTGCACCTCGGCGGCCCAGCCCTGTTCGGGCCCATCGGGATGGTGGTGGCCGCGGCGCTCAAGAGCGACATTCAGGAGT
CGCTCAACCGCTTCAAGCAGCTCTACGCCCCGTCCGCGACCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6867.59 Da
• Isoelectric Point: 4.6868

>AT21789 WP_011730673.1 NC_008596:c5720736-5720548 [Mycolicibacterium smegmatis MC2 155]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIHKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT21789 NC_008596:c5720736-5720548 [Mycolicibacterium smegmatis MC2 155]
ATGGGATTCCTGGACAAGGCCAAAGACCTCTTGTCGCAGAACGCCGACAAGGTGGACACCGTCATTGACAAGGCCGGCGA
CATGATCGACGAGAAGACGCAGGGCAAGTACGCCCAGCACGTCGACAAGGCGCAGGACGCCGCGAAGAACGCCATCCACA
AAGACGACCCTCAAGAACCACAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A653F9V2