Detailed information of TA system TA217845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3491451..3492229
• Replicon: chromosome
• Accession: NZ_CP083951
• Organism: Mesorhizobium sp. B2-8-5

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FJ430_RS16860
• Protein ID: WP_140705585.1
• Coordinates: 3491451..3491942 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FJ430_RS16865
• Protein ID: WP_140705583.1
• Coordinates: 3491939..3492229 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJ430_RS16835 (FJ430_16835)	3486795..3487736	+	942	WP_140705593.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FJ430_RS16840 (FJ430_16840)	3487816..3488658	+	843	WP_140705591.1	DUF1194 domain-containing protein	-
FJ430_RS16845 (FJ430_16845)	3488872..3489822	+	951	WP_140705589.1	ABC transporter substrate-binding protein	-
FJ430_RS16850 (FJ430_16850)	3489890..3490663	+	774	WP_140705990.1	ABC transporter permease	-
FJ430_RS16855 (FJ430_16855)	3490660..3491448	+	789	WP_140705587.1	ABC transporter ATP-binding protein	-
FJ430_RS16860 (FJ430_16860)	3491451..3491942	-	492	WP_140705585.1	GNAT family N-acetyltransferase	Toxin
FJ430_RS16865 (FJ430_16865)	3491939..3492229	-	291	WP_140705583.1	DUF1778 domain-containing protein	Antitoxin
FJ430_RS16870 (FJ430_16870)	3492295..3493296	-	1002	WP_140646604.1	GlxA family transcriptional regulator	-
FJ430_RS31630	3493539..3493673	-	135	WP_112099240.1	hypothetical protein	-
FJ430_RS16875 (FJ430_16875)	3494023..3494208	-	186	WP_140646602.1	hypothetical protein	-
FJ430_RS16880 (FJ430_16880)	3494428..3495327	+	900	WP_040981895.1	LysR substrate-binding domain-containing protein	-
FJ430_RS16885 (FJ430_16885)	3495329..3496294	-	966	WP_140705581.1	alpha/beta hydrolase	-
FJ430_RS16890 (FJ430_16890)	3496606..3496791	-	186	WP_181166902.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18175.88 Da
• Isoelectric Point: 9.8285

>T217845 WP_140705585.1 NZ_CP083951:c3491942-3491451 [Mesorhizobium sp. B2-8-5]
VNPFRIEKLARGHPVEKFDCGEQPLNRFLARYAFQNQQANASQTYIGLSSENVIGFYTLVVGEVAYDDAPDRLKKGLARH
PVPIMLLARLAVSLDWQGRGVGAGLLRDAVLRSLRAADIAGIRAITVHAKGDNARSFYERYGFIQSPTDPLHLYVLIKDL
RNL

Nucleotide

• Length: 492 bp

>T217845 NZ_CP114891:3109766-3110107 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10665.13 Da
• Isoelectric Point: 8.6351

>AT217845 WP_140705583.1 NZ_CP083951:c3492229-3491939 [Mesorhizobium sp. B2-8-5]
MATRTNRSEKLDLRLTAEAKRILATAAAAERRSLSDFVLESALGRAEETLADRRTFYLDAEKWAAFLSALDAPTQDLPRL
KKLLSEPSIFSGRDPA

Nucleotide

• Length: 291 bp

>AT217845 NZ_CP114891:3109428-3109745 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA