Detailed information of TA system TA217656

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 51851..52686
• Replicon: chromosome
• Accession: NZ_CP083877
• Organism: Escherichia coli strain NDM4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: LCE22_RS00270
• Protein ID: WP_000854735.1
• Coordinates: 51851..52228 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A7ZRI0
• Locus tag: LCE22_RS00275
• Protein ID: WP_012139188.1
• Coordinates: 52318..52686 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE22_RS00235 (46915)	46915..48099	-	1185	WP_001172898.1	sugar efflux transporter	-
LCE22_RS00240 (48496)	48496..48657	-	162	Protein_47	virulence RhuM family protein	-
LCE22_RS00245 (48895)	48895..49737	-	843	WP_033878271.1	DUF4942 domain-containing protein	-
LCE22_RS00250 (49822)	49822..50019	-	198	WP_000839281.1	DUF957 domain-containing protein	-
LCE22_RS00255 (50031)	50031..50381	-	351	Protein_50	DUF5983 family protein	-
LCE22_RS00260 (51303)	51303..51304	-	2	WP_086558215.1	IS3-like element IS2 family transposase	-
LCE22_RS00265 (51714)	51714..51854	-	141	Protein_52	DUF5983 family protein	-
LCE22_RS00270 (51851)	51851..52228	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
LCE22_RS00275 (52318)	52318..52686	-	369	WP_012139188.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LCE22_RS00280 (52766)	52766..52987	-	222	WP_033878268.1	DUF987 domain-containing protein	-
LCE22_RS00285 (53074)	53074..53550	-	477	WP_001186746.1	RadC family protein	-
LCE22_RS00290 (53566)	53566..54051	-	486	WP_000206653.1	antirestriction protein	-
LCE22_RS00295 (54143)	54143..54961	-	819	WP_213208608.1	DUF932 domain-containing protein	-
LCE22_RS00300 (55051)	55051..55284	-	234	WP_001278287.1	DUF905 family protein	-
LCE22_RS00305 (55290)	55290..55967	-	678	WP_001097301.1	hypothetical protein	-
LCE22_RS00310 (56115)	56115..56795	-	681	WP_001282914.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T217656 WP_000854735.1 NZ_CP083877:c52228-51851 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T217656 NZ_CP114551:c1015602-1015309 [Xylella fastidiosa subsp. pauca]
ATGACCGAGAAACTGACGAGCTACGATCCGGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCCGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTAGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTAGCC
GTGATGAAGGCGCTAGGGATCGAGCTCTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13582.31 Da
• Isoelectric Point: 6.2035

>AT217656 WP_012139188.1 NZ_CP083877:c52686-52318 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT217656 NZ_CP114551:c1015901-1015605 [Xylella fastidiosa subsp. pauca]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCACGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCAGCGTGGCGACACGATCTACTTGCTACTTTGCGGCGGTGACAAAGGA
TTACAAGCGCGTGACATCAAGACTGCGCTGCACCTCTCTGAACAATGGAGCGAATGA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A7ZRI0