Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 832880..833534 Replicon chromosome
Accession NZ_CP083872
Organism Escherichia coli strain NDM5

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LCE27_RS04090 Protein ID WP_000244781.1
Coordinates 833127..833534 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LCE27_RS04085 Protein ID WP_000354046.1
Coordinates 832880..833146 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCE27_RS04065 (828968) 828968..830401 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
LCE27_RS04070 (830446) 830446..830757 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
LCE27_RS04075 (830921) 830921..831580 + 660 WP_000250269.1 hemolysin III family protein -
LCE27_RS04080 (831657) 831657..832637 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
LCE27_RS04085 (832880) 832880..833146 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LCE27_RS04090 (833127) 833127..833534 + 408 WP_000244781.1 protein YgfX Toxin
LCE27_RS04095 (833574) 833574..834095 - 522 WP_001521233.1 flavodoxin FldB -
LCE27_RS04100 (834207) 834207..835103 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LCE27_RS04105 (835128) 835128..835838 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LCE27_RS04110 (835844) 835844..837577 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T217630 WP_000244781.1 NZ_CP083872:833127-833534 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T217630 NZ_CP114549:1200661-1200936 [Xylella fastidiosa subsp. pauca]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCGCGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAACCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGCGGCT
TTTGGCGTTATCGTGTTGGTGATTTCCGGGTCGTCTGCGCCATTCAAGATAGCGTTTTGCGCGTCCTTGTGGTGCGTGTT
GGTCACCGGGGTGAGATTTACCGACGAGAATGCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT217630 WP_000354046.1 NZ_CP083872:832880-833146 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT217630 NZ_CP114549:1200450-1200677 [Xylella fastidiosa subsp. pauca]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTGAACTATCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References