Detailed information of TA system TA217627

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 24411..25246
• Replicon: chromosome
• Accession: NZ_CP083872
• Organism: Escherichia coli strain NDM5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: LCE27_RS00145
• Protein ID: WP_000854735.1
• Coordinates: 24411..24788 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A7ZRI0
• Locus tag: LCE27_RS00150
• Protein ID: WP_012139188.1
• Coordinates: 24878..25246 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE27_RS00110 (19474)	19474..20658	-	1185	WP_001172898.1	sugar efflux transporter	-
LCE27_RS00115 (21055)	21055..21216	-	162	Protein_22	virulence RhuM family protein	-
LCE27_RS00120 (21454)	21454..22296	-	843	WP_033878271.1	DUF4942 domain-containing protein	-
LCE27_RS00125 (22381)	22381..22578	-	198	WP_000839281.1	DUF957 domain-containing protein	-
LCE27_RS00130 (22590)	22590..22940	-	351	Protein_25	DUF5983 family protein	-
LCE27_RS00140 (24274)	24274..24414	-	141	Protein_27	DUF5983 family protein	-
LCE27_RS00145 (24411)	24411..24788	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
LCE27_RS00150 (24878)	24878..25246	-	369	WP_012139188.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LCE27_RS00155 (25326)	25326..25547	-	222	WP_033878268.1	DUF987 domain-containing protein	-
LCE27_RS00160 (25634)	25634..26110	-	477	WP_001186746.1	RadC family protein	-
LCE27_RS00165 (26126)	26126..26611	-	486	WP_000206653.1	antirestriction protein	-
LCE27_RS00170 (26703)	26703..27521	-	819	WP_213208608.1	DUF932 domain-containing protein	-
LCE27_RS00175 (27611)	27611..27844	-	234	WP_001278287.1	DUF905 family protein	-
LCE27_RS00180 (27850)	27850..28527	-	678	WP_001097301.1	hypothetical protein	-
LCE27_RS00185 (28675)	28675..29355	-	681	WP_001282914.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T217627 WP_000854735.1 NZ_CP083872:c24788-24411 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T217627 NZ_CP114549:997619-997915 [Xylella fastidiosa subsp. pauca]
ATGCCACGGGTGATCTTCGCTCCCGAAGCCATCCTGAACATTCAACGATTGCGGAATTTCCTGCATCCGAAGAACACCGA
TGCCGCAAGGCGGGCCGGTGAAGCCATCATGCGGGGTGCGCGGATGCTTGGAGCGCAGCCACATATCGGTCGTCCGGTTG
ATGATATGCCCGATGAGTACAGGGAATGGCTGATTGACTTCGGAGATAGCGGTTATGTGGCCCGCTACCACATTGATGGC
GATACCGTCACGATCTTGGCTGTTCGACACCATAAGGAAGTGGGATACACCAGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13582.31 Da
• Isoelectric Point: 6.2035

>AT217627 WP_012139188.1 NZ_CP083872:c25246-24878 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT217627 NZ_CP114549:997353-997631 [Xylella fastidiosa subsp. pauca]
ATGGGCATGGCAACCTCCATCAAGCTTGATGATGAGCTGAAAGGACGTGTGCAGCATCTGGCCGATGTTCGCCGCCGCAC
TTCGCACTGGATCATGCGCGAGGCGATCGCGCAGTACGTTGAGCGTGAGGAAAAGCGTGAGACGTTTCGGCAGGACACGC
TGAAGGCATGGGAAGAGTTTCAGGCGACCGGCCTGCACGTGACGACCGAAGAAGTCGAGAAGTGGCTTTCGAGTTGGGGC
ACTGAAAATGAATTGTCAGCGCCCGTATGCCACGGGTGA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A7ZRI0