Detailed information of TA system TA217605

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 51852..52687
• Replicon: chromosome
• Accession: NZ_CP083869
• Organism: Escherichia coli strain NDM6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: LCE24_RS01615
• Protein ID: WP_000854735.1
• Coordinates: 51852..52229 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A7ZRI0
• Locus tag: LCE24_RS01620
• Protein ID: WP_012139188.1
• Coordinates: 52319..52687 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCE24_RS01580 (46915)	46915..48099	-	1185	WP_001172898.1	sugar efflux transporter	-
LCE24_RS01585 (48496)	48496..48657	-	162	Protein_47	virulence RhuM family protein	-
LCE24_RS01590 (48895)	48895..49737	-	843	WP_033878271.1	DUF4942 domain-containing protein	-
LCE24_RS01595 (49822)	49822..50019	-	198	WP_000839281.1	DUF957 domain-containing protein	-
LCE24_RS01600 (50031)	50031..50381	-	351	Protein_50	DUF5983 family protein	-
LCE24_RS01610 (51715)	51715..51855	-	141	Protein_52	DUF5983 family protein	-
LCE24_RS01615 (51852)	51852..52229	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
LCE24_RS01620 (52319)	52319..52687	-	369	WP_012139188.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
LCE24_RS01625 (52767)	52767..52988	-	222	WP_033878268.1	DUF987 domain-containing protein	-
LCE24_RS01630 (53075)	53075..53551	-	477	WP_001186746.1	RadC family protein	-
LCE24_RS01635 (53567)	53567..54052	-	486	WP_000206653.1	antirestriction protein	-
LCE24_RS01640 (54144)	54144..54962	-	819	WP_213208608.1	DUF932 domain-containing protein	-
LCE24_RS01645 (55052)	55052..55285	-	234	WP_001278287.1	DUF905 family protein	-
LCE24_RS01650 (55291)	55291..55968	-	678	WP_001097301.1	hypothetical protein	-
LCE24_RS01655 (56116)	56116..56796	-	681	WP_001282914.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T217605 WP_000854735.1 NZ_CP083869:c52229-51852 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T217605 NZ_CP114516:124011-124262 [Ligilactobacillus salivarius]
ATGAATAAGAATTGGTCTGATGAAGCCTGGGATGACTACATGAGCTGGCAAAGTGATAAAAGAATAGTTAAGAAAATTAA
TAAGCTAATAAAAGATATTGATAGACATCCTTTTTCAGGAATAGGTAAACCAGAACCGTTAAAATATGGTTTATCAGGAA
CATGGTCTAGAAGAATTACGGAAGAGCATCGAATAGTCTATGAGATAAAAGATGATGAGATACGTATCTATTCTGTCAGA
GACCATTATTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13582.31 Da
• Isoelectric Point: 6.2035

>AT217605 WP_012139188.1 NZ_CP083869:c52687-52319 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT217605 NZ_CP114516:123752-124018 [Ligilactobacillus salivarius]
ATGGAAGCTACAACATACTCTAATTTTAGAAAATCCCTTAAATCATTCATGAAACAAGTCAATGAAGATTCAGAACCTTT
AATTGTTACTAATAAAAATAAAGAAGAAGATATTGTTGTTATGGGTAAAGATGACTATGATTCGCTAGTCGAAACCATGA
GAATTATAGAAAATAAATATCTCTATAACAAGATAAGACGTGGCAGAGAACAATTAGATTCTAAAAGTAACTACCACGAT
TTAATCGAGGTTGATGGCGATGAATAA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A7ZRI0