Detailed information of TA system TA217578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 93050..94673
• Replicon: plasmid p12379_1
• Accession: NZ_CP083858
• Organism: Enterobacter kobei strain 12379-yvys

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: LCD50_RS22410
• Protein ID: WP_000088044.1
• Coordinates: 93348..94673 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: LCD50_RS22405
• Protein ID: WP_012477377.1
• Coordinates: 93050..93343 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD50_RS22385 (LCD50_22425)	88849..89241	+	393	WP_000341066.1	hypothetical protein	-
LCD50_RS22390 (LCD50_22430)	89900..90556	+	657	WP_000462754.1	trypsin-like serine protease	-
LCD50_RS22395 (LCD50_22435)	90759..91256	+	498	WP_000340139.1	membrane protein	-
LCD50_RS22400 (LCD50_22440)	91261..92649	+	1389	WP_012695445.1	hypothetical protein	-
LCD50_RS22405 (LCD50_22445)	93050..93343	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
LCD50_RS22410 (LCD50_22450)	93348..94673	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
LCD50_RS22415 (LCD50_22455)	94734..94940	+	207	WP_000134171.1	hypothetical protein	-
LCD50_RS22420 (LCD50_22460)	95041..95451	+	411	WP_000985909.1	hypothetical protein	-
LCD50_RS22425 (LCD50_22465)	95464..95988	+	525	WP_001805097.1	hypothetical protein	-
LCD50_RS22430 (LCD50_22470)	96170..97174	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / aadA2 / qacE / sul1 / blaCTX-M-9 / mcr-9	-	1..248708	248708

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T217578 WP_000088044.1 NZ_CP083858:93348-94673 [Enterobacter kobei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T217578 NZ_CP114374:c5468242-5467964 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT217578 WP_012477377.1 NZ_CP083858:93050-93343 [Enterobacter kobei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT217578 NZ_CP114374:c5467952-5467647 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7