Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1047829..1048449 Replicon chromosome
Accession NZ_CP083857
Organism Enterobacter kobei strain 12379-yvys

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag LCD50_RS04950 Protein ID WP_014882802.1
Coordinates 1047829..1048047 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag LCD50_RS04955 Protein ID WP_008499288.1
Coordinates 1048075..1048449 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD50_RS04920 (LCD50_04930) 1043838..1044098 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
LCD50_RS04925 (LCD50_04935) 1044101..1044241 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LCD50_RS04930 (LCD50_04940) 1044238..1044948 - 711 WP_014882798.1 GNAT family protein -
LCD50_RS04935 (LCD50_04945) 1045050..1046510 + 1461 WP_014882799.1 PLP-dependent aminotransferase family protein -
LCD50_RS04940 (LCD50_04950) 1046482..1046949 - 468 WP_014882800.1 YlaC family protein -
LCD50_RS04945 (LCD50_04955) 1047068..1047619 - 552 WP_014882801.1 maltose O-acetyltransferase -
LCD50_RS04950 (LCD50_04960) 1047829..1048047 - 219 WP_014882802.1 HHA domain-containing protein Toxin
LCD50_RS04955 (LCD50_04965) 1048075..1048449 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
LCD50_RS04960 (LCD50_04970) 1048961..1052107 - 3147 WP_247170711.1 multidrug efflux RND transporter permease subunit AcrB -
LCD50_RS04965 (LCD50_04975) 1052130..1053323 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T217567 WP_014882802.1 NZ_CP083857:c1048047-1047829 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T217567 NZ_CP114365:c28190-27939 [Klebsiella variicola]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT217567 WP_008499288.1 NZ_CP083857:c1048449-1048075 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT217567 NZ_CP114365:c27952-27725 [Klebsiella variicola]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References