Detailed information of TA system TA217565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 63302..63948
• Replicon: chromosome
• Accession: NZ_CP083857
• Organism: Enterobacter kobei strain 12379-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LCD50_RS00325
• Protein ID: WP_063434484.1
• Coordinates: 63598..63948 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCD50_RS00320
• Protein ID: WP_063614463.1
• Coordinates: 63302..63601 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD50_RS00290 (LCD50_00295)	58718..59677	-	960	WP_023333995.1	DUF523 and DUF1722 domain-containing protein	-
LCD50_RS00295 (LCD50_00300)	59779..60189	-	411	WP_014881954.1	hydroxyisourate hydrolase	-
LCD50_RS00300 (LCD50_00305)	60289..61014	-	726	WP_014881955.1	MerR family transcriptional regulator	-
LCD50_RS00305 (LCD50_00310)	61132..61656	+	525	WP_014881956.1	lipocalin family protein	-
LCD50_RS00310 (LCD50_00315)	61735..62733	+	999	WP_179120407.1	class I SAM-dependent methyltransferase	-
LCD50_RS00315 (LCD50_00320)	62835..63272	+	438	WP_063614464.1	acetyltransferase	-
LCD50_RS00320 (LCD50_00325)	63302..63601	-	300	WP_063614463.1	XRE family transcriptional regulator	Antitoxin
LCD50_RS00325 (LCD50_00330)	63598..63948	-	351	WP_063434484.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LCD50_RS00330 (LCD50_00335)	64196..64393	-	198	Protein_65	hydroxyacid dehydrogenase	-
LCD50_RS00335 (LCD50_00340)	64498..64839	-	342	WP_213783223.1	SymE family type I addiction module toxin	-
LCD50_RS00340 (LCD50_00345)	64965..65156	+	192	WP_213783222.1	hypothetical protein	-
LCD50_RS00345 (LCD50_00350)	65268..66686	-	1419	WP_244997000.1	antiviral reverse transcriptase Drt3b	-
LCD50_RS00350 (LCD50_00355)	67632..68456	-	825	WP_247170655.1	antiviral reverse transcriptase Drt3a	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.52 Da
• Isoelectric Point: 6.9811

>T217565 WP_063434484.1 NZ_CP083857:c63948-63598 [Enterobacter kobei]
MWTVLFGKVFELWLLEQEEGLQEKVLADMLNLQNYGPRLPRPYADTVKGSQYNNMKELRIQYAGRPIRALFAFDPERQAI
VLCAGNKSNDKAFYEKLIRIADDEFTRHLTSLETAK

Nucleotide

• Length: 351 bp

>T217565 NZ_CP114363:c14256-13906 [Klebsiella variicola]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11190.06 Da
• Isoelectric Point: 6.9842

>AT217565 WP_063614463.1 NZ_CP083857:c63601-63302 [Enterobacter kobei]
VKTLNELITSRSPESQQRIKEMAEEMILETGLQLLREELRLSQKNVAELMGVSQPAVTQIEQRGNELKIATLKRYIEAMG
GKLCIDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT217565 NZ_CP114363:c13901-13599 [Klebsiella variicola]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA