Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31791..32446 Replicon chromosome
Accession NZ_CP083857
Organism Enterobacter kobei strain 12379-yvys

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LCD50_RS00150 Protein ID WP_244997003.1
Coordinates 31791..32030 (+) Length 80 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LCD50_RS00155 Protein ID WP_023331687.1
Coordinates 32027..32446 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD50_RS00130 (LCD50_00130) 27381..28469 + 1089 WP_014881924.1 cellulase family glycosylhydrolase -
LCD50_RS00135 (LCD50_00135) 28372..29556 - 1185 WP_045269345.1 multidrug efflux MFS transporter EmrD -
LCD50_RS00140 (LCD50_00140) 29732..30565 - 834 WP_045134488.1 EamA family transporter -
LCD50_RS00145 (LCD50_00145) 30628..31074 - 447 WP_014881927.1 GNAT family N-acetyltransferase -
LCD50_RS00150 (LCD50_00155) 31791..32030 + 240 WP_244997003.1 type II toxin-antitoxin system HigB family toxin Toxin
LCD50_RS00155 (LCD50_00160) 32027..32446 + 420 WP_023331687.1 helix-turn-helix domain-containing protein Antitoxin
LCD50_RS00160 (LCD50_00165) 32596..32694 + 99 WP_023331688.1 ilvB operon leader peptide IvbL -
LCD50_RS00165 (LCD50_00170) 32801..34489 + 1689 WP_213783232.1 acetolactate synthase large subunit -
LCD50_RS00170 (LCD50_00175) 34493..34780 + 288 WP_010426528.1 acetolactate synthase small subunit -
LCD50_RS00175 (LCD50_00180) 34863..35456 + 594 WP_014881931.1 transcriptional regulator UhpA -
LCD50_RS00180 (LCD50_00185) 35453..36958 + 1506 WP_045134486.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(3-68)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 80 a.a.        Molecular weight: 9493.07 Da        Isoelectric Point: 10.4651

>T217564 WP_244997003.1 NZ_CP083857:31791-32030 [Enterobacter kobei]
MGRTIEKSHCPTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKFYVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 240 bp

>T217564 NZ_CP114362:67656-68138 [Klebsiella variicola]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15561.18 Da        Isoelectric Point: 5.6761

>AT217564 WP_023331687.1 NZ_CP083857:32027-32446 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT217564 NZ_CP114362:67402-67668 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References