Detailed information of TA system TA217539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3154345..3155075
• Replicon: chromosome
• Accession: NZ_CP083849
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: LCD40_RS15390
• Protein ID: WP_023302825.1
• Coordinates: 3154761..3155075 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LCD40_RS15385
• Protein ID: WP_032620773.1
• Coordinates: 3154345..3154761 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LCD40_RS15360 (LCD40_15380)	3149793..3151298	-	1506	WP_017694010.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
LCD40_RS15365 (LCD40_15385)	3151295..3151888	-	594	WP_003861054.1	transcriptional regulator UhpA	-
LCD40_RS15370 (LCD40_15390)	3152014..3152301	-	288	WP_015570113.1	acetolactate synthase small subunit	-
LCD40_RS15375 (LCD40_15395)	3152305..3153993	-	1689	WP_023302827.1	acetolactate synthase large subunit	-
LCD40_RS15380 (LCD40_15400)	3154100..3154198	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
LCD40_RS15385 (LCD40_15405)	3154345..3154761	-	417	WP_032620773.1	helix-turn-helix domain-containing protein	Antitoxin
LCD40_RS15390 (LCD40_15410)	3154761..3155075	-	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
LCD40_RS15395 (LCD40_15415)	3155577..3155651	+	75	WP_223237555.1	type I toxin-antitoxin system toxin TisB	-
LCD40_RS15400 (LCD40_15420)	3155716..3156162	+	447	WP_003861064.1	GNAT family N-acetyltransferase	-
LCD40_RS15405 (LCD40_15425)	3156225..3157058	+	834	WP_015570117.1	DMT family transporter	-
LCD40_RS15410 (LCD40_15430)	3157234..3158418	+	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
LCD40_RS15415 (LCD40_15435)	3158324..3159409	-	1086	Protein_3022	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T217539 WP_023302825.1 NZ_CP083849:c3155075-3154761 [Enterobacter hormaechei subsp. steigerwaltii]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T217539 NZ_CP114354:795335-795745 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTCCCCTGGCCGCTGAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGCACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15249.77 Da
• Isoelectric Point: 5.2068

>AT217539 WP_032620773.1 NZ_CP083849:c3154761-3154345 [Enterobacter hormaechei subsp. steigerwaltii]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 417 bp

>AT217539 NZ_CP114354:795088-795354 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2