Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743913..744652 Replicon chromosome
Accession NZ_CP083849
Organism Enterobacter hormaechei subsp. steigerwaltii strain 14269-yvys

Toxin (Protein)


Gene name tacT Uniprot ID A0A822W909
Locus tag LCD40_RS03710 Protein ID WP_017382345.1
Coordinates 744167..744652 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag LCD40_RS03705 Protein ID WP_003857131.1
Coordinates 743913..744179 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD40_RS03685 (LCD40_03690) 739389..740375 + 987 WP_017382341.1 DUF979 domain-containing protein -
LCD40_RS03690 (LCD40_03695) 740385..741380 + 996 WP_023303746.1 DUF2891 domain-containing protein -
LCD40_RS03695 (LCD40_03700) 741402..742790 - 1389 WP_032619651.1 MFS transporter -
LCD40_RS03700 (LCD40_03705) 742920..743849 + 930 WP_023303744.1 LysR family transcriptional regulator -
LCD40_RS03705 (LCD40_03710) 743913..744179 + 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
LCD40_RS03710 (LCD40_03715) 744167..744652 + 486 WP_017382345.1 GNAT family N-acetyltransferase Toxin
LCD40_RS03715 (LCD40_03720) 744703..745272 - 570 WP_017382346.1 hypothetical protein -
LCD40_RS03720 (LCD40_03725) 745626..746240 - 615 WP_023303743.1 NUDIX hydrolase -
LCD40_RS03725 (LCD40_03730) 746240..747532 - 1293 WP_023303742.1 glycosyl hydrolase family 28 protein -
LCD40_RS03730 (LCD40_03735) 747637..748395 - 759 WP_023303741.1 DeoR/GlpR family DNA-binding transcription regulator -
LCD40_RS03735 (LCD40_03740) 748482..749066 + 585 WP_017382349.1 GDP-mannose pyrophosphatase nudK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17510.21 Da        Isoelectric Point: 9.9658

>T217537 WP_017382345.1 NZ_CP083849:744167-744652 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARAFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T217537 NZ_CP114354:342415-342783 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGACGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGTGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT217537 WP_003857131.1 NZ_CP083849:743913-744179 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT217537 NZ_CP114354:342197-342418 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATCAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822W909

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References