Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26374..27017 Replicon plasmid p13840_1
Accession NZ_CP083820
Organism Enterobacter roggenkampii strain 13840-yvys

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag LCD41_RS23050 Protein ID WP_000754567.1
Coordinates 26374..26790 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag LCD41_RS23055 Protein ID WP_023293777.1
Coordinates 26787..27017 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCD41_RS23020 (LCD41_23070) 21560..22141 + 582 WP_008502230.1 TetR/AcrR family transcriptional regulator -
LCD41_RS23025 (LCD41_23075) 22146..22484 + 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
LCD41_RS23030 (LCD41_23080) 22514..22843 - 330 WP_008502229.1 thioredoxin family protein -
LCD41_RS23035 (LCD41_23085) 23056..24162 + 1107 WP_247170006.1 alkene reductase -
LCD41_RS23040 (LCD41_23090) 24228..24929 + 702 WP_008502228.1 DsbA family oxidoreductase -
LCD41_RS23045 (LCD41_23095) 25055..26023 + 969 WP_247170007.1 IS5-like element IS903B family transposase -
LCD41_RS23050 (LCD41_23100) 26374..26790 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
LCD41_RS23055 (LCD41_23105) 26787..27017 - 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LCD41_RS23060 (LCD41_23110) 27646..28350 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
LCD41_RS23065 (LCD41_23115) 28442..29077 - 636 WP_000677445.1 type 3 fimbria minor subunit MrkF -
LCD41_RS23070 (LCD41_23120) 29091..30086 - 996 WP_134866775.1 type 3 fimbria adhesin subunit MrkD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 mrkF / mrkD / mrkC / mrkB / mrkA 1..94721 94721
- inside IScluster/Tn mcr-10 - 2813..28350 25537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T217442 WP_000754567.1 NZ_CP083820:c26790-26374 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T217442 NZ_CP114308:2222200-2222303 [Klebsiella oxytoca]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT217442 WP_023293777.1 NZ_CP083820:c27017-26787 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT217442 NZ_CP114308:c2222310-2222165 [Klebsiella oxytoca]
AAAAGATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References