Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 495820..496438 Replicon chromosome
Accession NC_008563
Organism Escherichia coli APEC O1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag APECO1_RS02335 Protein ID WP_001291435.1
Coordinates 495820..496038 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag APECO1_RS02340 Protein ID WP_000344800.1
Coordinates 496064..496438 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO1_RS02305 491107..491679 + 573 WP_000779831.1 YbaY family lipoprotein -
APECO1_RS02310 491710..492021 - 312 WP_000409908.1 MGMT family protein -
APECO1_RS02315 492400..492753 + 354 WP_000878140.1 DUF1428 domain-containing protein -
APECO1_RS02320 492795..494345 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
APECO1_RS02325 494509..494979 - 471 WP_000136192.1 YlaC family protein -
APECO1_RS02330 495095..495646 - 552 WP_000102543.1 maltose O-acetyltransferase -
APECO1_RS02335 495820..496038 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
APECO1_RS02340 496064..496438 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
APECO1_RS02345 496983..500132 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
APECO1_RS02350 500155..501348 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T21744 WP_001291435.1 NC_008563:c496038-495820 [Escherichia coli APEC O1]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T21744 NC_008563:c496038-495820 [Escherichia coli APEC O1]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT21744 WP_000344800.1 NC_008563:c496438-496064 [Escherichia coli APEC O1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT21744 NC_008563:c496438-496064 [Escherichia coli APEC O1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References