Detailed information of TA system TA217369

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4213787..4214493
• Replicon: chromosome
• Accession: NZ_CP083796
• Organism: Klebsiella pneumoniae strain INF019 isolate INF019

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: LB481_RS20865
• Protein ID: WP_024622903.1
• Coordinates: 4214125..4214493 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: LB481_RS20860
• Protein ID: WP_023302278.1
• Coordinates: 4213787..4214104 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LB481_RS20815 (4208928)	4208928..4209752	+	825	WP_023302271.1	DUF932 domain-containing protein	-
LB481_RS20820 (4209961)	4209961..4210671	+	711	WP_023302272.1	DeoR family transcriptional regulator	-
LB481_RS20825 (4210697)	4210697..4211233	+	537	WP_023302273.1	DUF4339 domain-containing protein	-
LB481_RS20830 (4211275)	4211275..4211712	+	438	WP_023301392.1	hypothetical protein	-
LB481_RS20835 (4211779)	4211779..4212189	+	411	WP_023302274.1	hypothetical protein	-
LB481_RS20840 (4212267)	4212267..4212503	+	237	WP_032410024.1	DUF905 domain-containing protein	-
LB481_RS20845 (4212590)	4212590..4213048	+	459	WP_023302275.1	antirestriction protein	-
LB481_RS20850 (4213057)	4213057..4213539	+	483	WP_023302276.1	RadC family protein	-
LB481_RS20855 (4213548)	4213548..4213769	+	222	WP_023302277.1	DUF987 domain-containing protein	-
LB481_RS20860 (4213787)	4213787..4214104	+	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LB481_RS20865 (4214125)	4214125..4214493	+	369	WP_024622903.1	TA system toxin CbtA family protein	Toxin
LB481_RS20870 (4214490)	4214490..4214831	+	342	WP_024622904.1	hypothetical protein	-
LB481_RS20875 (4214954)	4214954..4217599	-	2646	WP_024622905.1	LuxR C-terminal-related transcriptional regulator	-
LB481_RS20880 (4217973)	4217973..4218932	+	960	WP_023302280.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.82 Da
• Isoelectric Point: 7.2897

>T217369 WP_024622903.1 NZ_CP083796:4214125-4214493 [Klebsiella pneumoniae]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRI

Nucleotide

• Length: 369 bp

>T217369 NZ_CP114168:c4472953-4471625 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTACCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTCTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGTACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCTGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAATCTATGGT
CACTCCCGTTTTTGCAACACCGATTTTGACGACAAGTTGGCTTGCTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT217369 WP_023302278.1 NZ_CP083796:4213787-4214104 [Klebsiella pneumoniae]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT217369 NZ_CP114168:c4473219-4472953 [Klebsiella variicola]
ATGACTTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTATCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACACTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTAGGGGTGGTGATGACGCTGAACGCCAAAGCCACAGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6