Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5394814..5395439 Replicon chromosome
Accession NZ_CP083775
Organism Klebsiella pneumoniae strain INF118 isolate INF118

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LB478_RS26800 Protein ID WP_004187928.1
Coordinates 5394814..5395197 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LB478_RS26805 Protein ID WP_004150355.1
Coordinates 5395197..5395439 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LB478_RS26785 (LB478_26785) 5392180..5393082 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LB478_RS26790 (LB478_26790) 5393079..5393714 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LB478_RS26795 (LB478_26795) 5393711..5394640 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LB478_RS26800 (LB478_26800) 5394814..5395197 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LB478_RS26805 (LB478_26805) 5395197..5395439 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LB478_RS26810 (LB478_26810) 5395644..5396561 + 918 WP_004178029.1 alpha/beta hydrolase -
LB478_RS26815 (LB478_26815) 5396575..5397516 - 942 WP_224440616.1 fatty acid biosynthesis protein FabY -
LB478_RS26820 (LB478_26820) 5397561..5397998 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LB478_RS26825 (LB478_26825) 5397995..5398855 - 861 WP_004146232.1 virulence factor BrkB family protein -
LB478_RS26830 (LB478_26830) 5398849..5399448 - 600 WP_023342375.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T217308 WP_004187928.1 NZ_CP083775:c5395197-5394814 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T217308 NZ_CP114134:c3713558-3713406 [Xanthomonas fragariae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCTTGTTTTCGGCCGGCGTGATGACTGGCTGTAACACCGTTGCTGGCGC
CGGCAAGGATATGCAACGCGCGGGCAGCAAGGTCGAAAAGAAGGCCGATAACTGCAGCGACGGTAAATGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT217308 WP_004150355.1 NZ_CP083775:c5395439-5395197 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT217308 NZ_CP114134:c3713325-3713191 [Xanthomonas fragariae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAATACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCTGGCGAGAAAGTGGAAGACGCTGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References