Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 58982..59718 | Replicon | plasmid pKSB1_9E_1 |
| Accession | NZ_CP083769 | ||
| Organism | Klebsiella pneumoniae strain KSB1_9E isolate KSB1_9E | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A2J5Q928 |
| Locus tag | LB480_RS26070 | Protein ID | WP_004098919.1 |
| Coordinates | 59236..59718 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | A0A455TK27 |
| Locus tag | LB480_RS26065 | Protein ID | WP_020804426.1 |
| Coordinates | 58982..59248 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LB480_RS26020 (LB480_26020) | 55164..55598 | + | 435 | WP_004118347.1 | hypothetical protein | - |
| LB480_RS26025 (LB480_26025) | 55778..55940 | - | 163 | Protein_61 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LB480_RS26030 (LB480_26030) | 56062..56241 | - | 180 | WP_004118349.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| LB480_RS26035 (LB480_26035) | 56555..56818 | + | 264 | WP_004118691.1 | hypothetical protein | - |
| LB480_RS26040 (LB480_26040) | 56815..57381 | + | 567 | WP_042922197.1 | hypothetical protein | - |
| LB480_RS26045 (LB480_26045) | 57412..57906 | + | 495 | WP_016528789.1 | hypothetical protein | - |
| LB480_RS26050 (LB480_26050) | 57967..58170 | + | 204 | WP_004150739.1 | HHA domain-containing protein | - |
| LB480_RS26055 (LB480_26055) | 58219..58476 | + | 258 | WP_004098928.1 | hypothetical protein | - |
| LB480_RS26060 (LB480_26060) | 58552..58806 | + | 255 | WP_032425571.1 | hypothetical protein | - |
| LB480_RS26065 (LB480_26065) | 58982..59248 | + | 267 | WP_020804426.1 | DUF1778 domain-containing protein | Antitoxin |
| LB480_RS26070 (LB480_26070) | 59236..59718 | + | 483 | WP_004098919.1 | GNAT family N-acetyltransferase | Toxin |
| LB480_RS26075 (LB480_26075) | 59919..61322 | + | 1404 | WP_001567368.1 | ISNCY-like element ISKpn21 family transposase | - |
| LB480_RS26080 (LB480_26080) | 61351..61983 | - | 633 | WP_001567369.1 | hypothetical protein | - |
| LB480_RS26085 (LB480_26085) | 62310..63290 | - | 981 | WP_000019450.1 | IS5-like element ISKpn26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..250893 | 250893 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17335.06 Da Isoelectric Point: 10.0704
>T217290 WP_004098919.1 NZ_CP083769:59236-59718 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T217290 NZ_CP114130:c2843614-2843240 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10124.47 Da Isoelectric Point: 4.3108
>AT217290 WP_020804426.1 NZ_CP083769:58982-59248 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV
Download Length: 267 bp
>AT217290 NZ_CP114130:c2844035-2843661 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J5Q928 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A455TK27 |