Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 69771..70507 Replicon plasmid pFAHZZU2591
Accession NZ_CP083752
Organism Klebsiella pneumoniae strain FAHZZU2591

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LBE00_RS25560 Protein ID WP_004098919.1
Coordinates 69771..70253 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LBE00_RS25565 Protein ID WP_004213599.1
Coordinates 70241..70507 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBE00_RS25540 (LBE00_25575) 65125..66471 + 1347 WP_011154555.1 dihydroorotase -
LBE00_RS25545 (LBE00_25580) 66535..67557 + 1023 WP_004214536.1 porphobilinogen synthase -
LBE00_RS25550 (LBE00_25585) 67732..68127 + 396 Protein_71 IS3 family transposase -
LBE00_RS25555 (LBE00_25590) 68123..69350 - 1228 Protein_72 IS3 family transposase -
LBE00_RS25560 (LBE00_25595) 69771..70253 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LBE00_RS25565 (LBE00_25600) 70241..70507 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LBE00_RS25570 (LBE00_25605) 70702..70932 - 231 WP_004213598.1 hypothetical protein -
LBE00_RS25575 (LBE00_25610) 70946..71149 - 204 WP_004213596.1 HHA domain-containing protein -
LBE00_RS25580 (LBE00_25615) 71210..71704 - 495 WP_004213594.1 hypothetical protein -
LBE00_RS25585 (LBE00_25620) 71746..74715 - 2970 WP_094936121.1 Tn3 family transposase -
LBE00_RS25590 (LBE00_25625) 74718..75275 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA / pla 1..202973 202973
- inside IScluster/Tn - - 68123..75275 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T217241 WP_004098919.1 NZ_CP083752:c70253-69771 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T217241 NZ_CP114115:c1855396-1855112 [Pseudomonas sp. GXZC]
ATGCAGGTTGAGTGGCTGAGGACAGCCCTCAGGAACCTTGACGATGAAGCCGCTTATATTGCTGAAGAAAATCCAAAAGC
TGCCCACGAATTTGTCCAGGAAATCTTGACTGGGCTCCAACAGATTGCTGAATTTCCAGCGATGGGAAAAGAGGGACGCG
TGCCAGGGACTCGCGAATGGGTCGTGCCTAGATGGCCCTACATTGTCCCGTATCGCATCCGAGGGGGCCGATTGCAAGTC
ATGCGGATCTTCCATACCCGGCGCCAGCCACCCGCATCCTGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT217241 WP_004213599.1 NZ_CP083752:c70507-70241 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT217241 NZ_CP114115:c1855625-1855386 [Pseudomonas sp. GXZC]
ATGTCTGTCATGTCCCTACGTATACCGGAAGATATCGCCGATACGCTTGCCAGTCTCGCCAAGGCCACCGGGCGCAGCAA
ATCCTTTCTGGCGGTTGACGCATTACGCGAATACCTCACGCGCGAAGCCTGGCAAATTGAGGAAATTCAAAAAGCACTAA
AAGAAGCGGATGCGGGCGATTTCGCTACCGCTGCCGAGGTAGAAGCTGTCGCTGATAAATGGCGCGCCAATGCAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References