Detailed information of TA system TA216790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3581184..3582021
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: LA372_RS17110
• Protein ID: WP_224204934.1
• Coordinates: 3581479..3582021 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A080JFU1
• Locus tag: LA372_RS17105
• Protein ID: WP_001577859.1
• Coordinates: 3581184..3581495 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS17080 (3576204)	3576204..3577151	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
LA372_RS17085 (3577173)	3577173..3579164	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LA372_RS17090 (3579154)	3579154..3579768	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LA372_RS17095 (3579768)	3579768..3580097	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LA372_RS17100 (3580109)	3580109..3580999	+	891	WP_000971336.1	heme o synthase	-
LA372_RS17105 (3581184)	3581184..3581495	+	312	WP_001577859.1	DUF1778 domain-containing protein	Antitoxin
LA372_RS17110 (3581479)	3581479..3582021	+	543	WP_224204934.1	GNAT family N-acetyltransferase	Toxin
LA372_RS17115 (3582077)	3582077..3583012	-	936	WP_045176456.1	tetratricopeptide repeat protein	-
LA372_RS17120 (3583419)	3583419..3584783	+	1365	WP_224204935.1	MFS transporter	-
LA372_RS17125 (3584911)	3584911..3585402	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
LA372_RS17130 (3585570)	3585570..3586481	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (65-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19834.10 Da
• Isoelectric Point: 8.8918

>T216790 WP_224204934.1 NZ_CP083585:3581479-3582021 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGKLIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T216790 NZ_CP113443:67424-67780 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11400.08 Da
• Isoelectric Point: 10.5548

>AT216790 WP_001577859.1 NZ_CP083585:3581184-3581495 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT216790 NZ_CP113443:67773-68075 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080JFU1