Detailed information of TA system TA216734

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4195774..4196145
• Replicon: chromosome
• Accession: NZ_CP083530
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: K9U05_RS19700
• Protein ID: WP_001317028.1
• Coordinates: 4195951..4196145 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4195774..4195952 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS19675 (4191729)	4191729..4193102	+	1374	WP_022645642.1	ATP-dependent RNA helicase DbpA	-
K9U05_RS19680 (4193231)	4193231..4194166	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
K9U05_RS19685 (4194218)	4194218..4195453	-	1236	WP_000040858.1	site-specific integrase	-
K9U05_RS19690 (4195455)	4195455..4195670	-	216	WP_000079604.1	excisionase XisR	-
- (4195774)	4195774..4195952	+	179	NuclAT_0	-	Antitoxin
- (4195774)	4195774..4195952	+	179	NuclAT_0	-	Antitoxin
- (4195774)	4195774..4195952	+	179	NuclAT_0	-	Antitoxin
- (4195774)	4195774..4195952	+	179	NuclAT_0	-	Antitoxin
K9U05_RS19695 (4195749)	4195749..4195958	-	210	WP_021565074.1	double-strand break reduction protein RcbA	-
K9U05_RS19700 (4195951)	4195951..4196145	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
K9U05_RS19705 (4196202)	4196202..4197011	-	810	WP_000166319.1	recombination protein RecT	-
K9U05_RS19710 (4197004)	4197004..4199604	-	2601	WP_228612492.1	exodeoxyribonuclease VIII	-
K9U05_RS19715 (4199706)	4199706..4199981	-	276	WP_000632297.1	protein RacC	-
K9U05_RS19720 (4200056)	4200056..4200226	-	171	WP_001352098.1	YdaE family protein	-
K9U05_RS19725 (4200226)	4200226..4200447	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4194218..4242836	48618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T216734 WP_001317028.1 NZ_CP083530:c4196145-4195951 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T216734 NZ_CP113256:513574-513924 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT216734 NZ_CP083530:4195774-4195952 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGAAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin