Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3365849..3366649 Replicon chromosome
Accession NZ_CP083497
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag K9U09_RS16090 Protein ID WP_000342449.1
Coordinates 3366122..3366649 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag K9U09_RS16085 Protein ID WP_001277108.1
Coordinates 3365849..3366115 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS16065 (3361507) 3361507..3362175 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
K9U09_RS16070 (3362168) 3362168..3363226 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
K9U09_RS16075 (3363471) 3363471..3364325 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
K9U09_RS16080 (3364596) 3364596..3365699 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
K9U09_RS16085 (3365849) 3365849..3366115 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
K9U09_RS16090 (3366122) 3366122..3366649 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
K9U09_RS16095 (3366646) 3366646..3367029 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
K9U09_RS16100 (3367453) 3367453..3368562 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
K9U09_RS16105 (3368610) 3368610..3369536 + 927 WP_228596619.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K9U09_RS16110 (3369533) 3369533..3370810 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
K9U09_RS16115 (3370807) 3370807..3371574 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T216616 WP_000342449.1 NZ_CP083497:3366122-3366649 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T216616 NZ_CP113187:28985-29290 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT216616 WP_001277108.1 NZ_CP083497:3365849-3366115 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT216616 NZ_CP113187:28765-28983 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References