Detailed information of TA system TA216382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2765781..2767218
• Replicon: chromosome
• Accession: NZ_CP083410
• Organism: Escherichia coli strain RP437

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: LAJ62_RS13535
• Protein ID: WP_000155570.1
• Coordinates: 2765781..2766854 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: LAJ62_RS13540
• Protein ID: WP_000461704.1
• Coordinates: 2766847..2767218 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LAJ62_RS13520 (2761214)	2761214..2763403	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
LAJ62_RS13525 (2763400)	2763400..2765016	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
LAJ62_RS13530 (2765376)	2765376..2765639	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
LAJ62_RS13535 (2765781)	2765781..2766854	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
LAJ62_RS13540 (2766847)	2766847..2767218	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
LAJ62_RS13545 (2767573)	2767573..2768436	+	864	WP_000787597.1	GTPase family protein	-
LAJ62_RS13550 (2768528)	2768528..2769349	+	822	WP_000197391.1	DUF932 domain-containing protein	-
LAJ62_RS13555 (2769566)	2769566..2770267	+	702	WP_000189409.1	WYL domain-containing protein	-
LAJ62_RS13560 (2770308)	2770308..2770544	+	237	WP_001144029.1	protein YpjK	-
LAJ62_RS13565 (2770544)	2770544..2770987	+	444	WP_000824222.1	lipoprotein YfjS	-
LAJ62_RS13570 (2771011)	2771011..2771478	+	468	WP_000702495.1	protein YfjT	-
LAJ62_RS13575 (2771865)	2771865..2772017	-	153	Protein_2657	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T216382 WP_000155570.1 NZ_CP083410:2765781-2766854 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T216382 NZ_CP113032:c2239878-2239516 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT216382 WP_000461704.1 NZ_CP083410:2766847-2767218 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT216382 NZ_CP113032:c2240045-2239875 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4