Detailed information of TA system TA216328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 835179..835714
• Replicon: plasmid pT173e
• Accession: NZ_CP083371
• Organism: Ensifer canadensis strain T173

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J3R84_RS23470
• Protein ID: WP_203529408.1
• Coordinates: 835179..835454 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J3R84_RS23475
• Protein ID: WP_203529406.1
• Coordinates: 835454..835714 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3R84_RS23450 (J3R84_23370)	831130..831807	+	678	WP_057204573.1	response regulator transcription factor	-
J3R84_RS23455 (J3R84_23375)	831804..833183	+	1380	WP_203529411.1	sensor histidine kinase	-
J3R84_RS23460 (J3R84_23380)	833582..834202	+	621	WP_203529409.1	NUDIX domain-containing protein	-
J3R84_RS23465 (J3R84_23385)	834565..834873	-	309	WP_203529430.1	hypothetical protein	-
J3R84_RS23470 (J3R84_23390)	835179..835454	-	276	WP_203529408.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3R84_RS23475 (J3R84_23395)	835454..835714	-	261	WP_203529406.1	ribbon-helix-helix domain-containing protein	Antitoxin
J3R84_RS23480 (J3R84_23400)	836210..836434	-	225	WP_057204567.1	hypothetical protein	-
J3R84_RS23485 (J3R84_23405)	836992..837614	+	623	Protein_774	DUF1073 domain-containing protein	-
J3R84_RS23490 (J3R84_23410)	838032..838349	+	318	WP_225906481.1	DUF2213 domain-containing protein	-
J3R84_RS23495 (J3R84_23415)	838828..839034	-	207	Protein_776	type II toxin-antitoxin system RelE/ParE family toxin	-
J3R84_RS23500 (J3R84_23420)	839132..839953	-	822	WP_203529398.1	universal stress protein	-
J3R84_RS23505 (J3R84_23425)	840291..840626	+	336	WP_203529396.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fcl / gmd	1..1913930	1913930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10715.38 Da
• Isoelectric Point: 10.8916

>T216328 WP_203529408.1 NZ_CP083371:c835454-835179 [Ensifer canadensis]
MQVNWTSKTLGDLARPFEFLAPVNRRAAARTVQPLTTAPTRLVEQPRLGEKLEEFAAREVRRILVGHYEIRYEIQQSIIY
VLRVWHTREAR

Nucleotide

• Length: 276 bp

>T216328 NZ_CP113015:c2671101-2670949 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9637.00 Da
• Isoelectric Point: 4.7640

>AT216328 WP_203529406.1 NZ_CP083371:c835714-835454 [Ensifer canadensis]
MKTKVLTAHVPLPLAERVDQLASRLERSRGWIIKQALTAWVDQEDERRLLTLEALADVDAGQVIDHQAVQAWADSLDNDK
PLSVPL

Nucleotide

• Length: 261 bp

>AT216328 NZ_CP113015:c2671725-2671126 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA