Detailed information of TA system TA216314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 152157..152662
• Replicon: chromosome
• Accession: NZ_CP083366
• Organism: Pseudomonas aeruginosa strain PS1793

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: FLI66_RS00710
• Protein ID: WP_003083773.1
• Coordinates: 152157..152438 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: FLI66_RS00715
• Protein ID: WP_003083775.1
• Coordinates: 152435..152662 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLI66_RS00685 (FLI66_00685)	147408..148757	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
FLI66_RS00690 (FLI66_00690)	148806..149492	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
FLI66_RS00695 (FLI66_00695)	149593..150327	+	735	WP_003110658.1	GntR family transcriptional regulator	-
FLI66_RS00700 (FLI66_00700)	150507..150917	+	411	WP_003110659.1	aegerolysin family protein	-
FLI66_RS00705 (FLI66_00705)	150949..151857	-	909	WP_003083769.1	LysR family transcriptional regulator	-
FLI66_RS00710 (FLI66_00710)	152157..152438	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
FLI66_RS00715 (FLI66_00715)	152435..152662	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
FLI66_RS00720 (FLI66_00720)	152838..153461	-	624	WP_003137011.1	hypothetical protein	-
FLI66_RS00725 (FLI66_00725)	153562..154062	+	501	WP_003101228.1	LEA type 2 family protein	-
FLI66_RS00730 (FLI66_00730)	154134..154475	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
FLI66_RS00735 (FLI66_00735)	154557..155984	-	1428	WP_003083784.1	GABA permease	-
FLI66_RS00740 (FLI66_00740)	156153..157646	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T216314 WP_003083773.1 NZ_CP083366:c152438-152157 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T216314 NZ_CP113009:c1836352-1835957 [Staphylococcus aureus]
ATGCAAAACATTAAATATTTAACTGAAAAACAAGTAATTGCCATTAATGTAAAAGCAATTCAAGAACTCTCACCTAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAGGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTTGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCGGCTTTTATATACAGAAATATAGCTCAAAAACATGCATTCTTTAATGCGAATAAGAGA
ACAGCTTTTACATCTATGGTTATTTTCTTGAAGTTAAATAAGATTAATTTTAATTGTACTCAAGACGAAGCGGTACAATT
TACTTTAAAAGTGGTAGAGGATAAAACTCTTACACTTGAAGATATTGCAGATTGGATCAAAAGGCATTGTAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT216314 WP_003083775.1 NZ_CP083366:c152662-152435 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT216314 NZ_CP113009:c1836582-1836352 [Staphylococcus aureus]
ATGGTTACTACAAGAAAACTAAGAAGAGCTGGCAATAGTTCAGTTGTTTCTGTACCAACAGAAGTGATCGCAGCACTCGG
AGTTACAAATGGTGACAATCTTAAATTTAATATTGAAGATAATAAAGTAACCATTGAAAAGGAGGTAAACGAAGACGAAG
AGTTTTTCAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTCTGAGAAAGTTGGTTGACATATAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9