Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1585401..1586137 Replicon chromosome
Accession NZ_CP083075
Organism Klebsiella pneumoniae strain 0113481141

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag K9F42_RS07865 Protein ID WP_032433360.1
Coordinates 1585655..1586137 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K9F42_RS07860 Protein ID WP_003026799.1
Coordinates 1585401..1585667 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F42_RS07835 (1581047) 1581047..1582186 + 1140 WP_032433368.1 mannitol dehydrogenase -
K9F42_RS07840 (1582215) 1582215..1582877 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
K9F42_RS07845 (1582861) 1582861..1583865 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
K9F42_RS07850 (1583883) 1583883..1584515 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
K9F42_RS07855 (1584525) 1584525..1585088 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
K9F42_RS07860 (1585401) 1585401..1585667 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K9F42_RS07865 (1585655) 1585655..1586137 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
K9F42_RS07870 (1586498) 1586498..1587097 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
K9F42_RS07875 (1587310) 1587310..1588254 - 945 WP_077254249.1 fimbrial protein -
K9F42_RS07880 (1588266) 1588266..1588844 - 579 WP_032432061.1 type 1 fimbrial protein -
K9F42_RS07885 (1588848) 1588848..1589588 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T216130 WP_032433360.1 NZ_CP083075:1585655-1586137 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T216130 NZ_CP112887:1899073-1899405 [Raoultella electrica]
ATGGACCGAGGGGAAATCTGGCTGGTTTCACTGGACCCTATTGCCGGTCACGAGCAATGCGGTAAACGTCCGGTGCTCAT
CGTTTCACAGGCATCGTTTAACAAGCTGACCCGGCTTCCGGTAGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCTCGCA
CGGCAGGCTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACGATTGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAACGCATACCTGACGCAGTTGTAAATGAAGTGCTTGCCAGGCTGGATGC
TATTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT216130 WP_003026799.1 NZ_CP083075:1585401-1585667 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT216130 NZ_CP112887:1898815-1899072 [Raoultella electrica]
ATGTATACGACTCGTCTTAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References