Detailed information of TA system TA216025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 175103..175625
• Replicon: plasmid p1-400195-1-18
• Accession: NZ_CP083048
• Organism: Klebsiella pneumoniae strain 400195-1-18

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A0C7KG00
• Locus tag: K9F24_RS27340
• Protein ID: WP_038991638.1
• Coordinates: 175341..175625 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: K9F24_RS27335
• Protein ID: WP_004181777.1
• Coordinates: 175103..175351 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9F24_RS27315 (K9F24_27255)	170312..171133	-	822	WP_004181772.1	hypothetical protein	-
K9F24_RS27320 (K9F24_27260)	171195..171548	-	354	WP_004181774.1	hypothetical protein	-
K9F24_RS27325 (K9F24_27265)	171693..172679	-	987	WP_040120328.1	hypothetical protein	-
K9F24_RS27330 (K9F24_27270)	173013..174812	-	1800	WP_043907032.1	ATP-dependent helicase	-
K9F24_RS27335 (K9F24_27275)	175103..175351	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K9F24_RS27340 (K9F24_27280)	175341..175625	+	285	WP_038991638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9F24_RS27345 (K9F24_27285)	175642..175743	-	102	Protein_190	IS200/IS605 family transposase	-
K9F24_RS27350 (K9F24_27290)	175779..177005	+	1227	WP_080030933.1	RNA-guided endonuclease TnpB family protein	-
K9F24_RS27355 (K9F24_27295)	177277..177501	-	225	Protein_192	transposase	-
K9F24_RS27360 (K9F24_27300)	177580..178008	-	429	WP_077256229.1	IS200/IS605 family transposase	-
K9F24_RS27365 (K9F24_27305)	178044..179231	+	1188	WP_043907031.1	RNA-guided endonuclease TnpB family protein	-
K9F24_RS27370	179276..179647	-	372	WP_044816264.1	hypothetical protein	-
K9F24_RS27375	179644..179988	-	345	WP_014386518.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	-	1..254008	254008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10993.73 Da
• Isoelectric Point: 10.6516

>T216025 WP_038991638.1 NZ_CP083048:175341-175625 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Nucleotide

• Length: 285 bp

>T216025 NZ_CP111034:c5367762-5367484 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT216025 WP_004181777.1 NZ_CP083048:175103-175351 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT216025 NZ_CP111034:c5367472-5367167 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0C7KG00

Antitoxin

• AlphaFold DB: A0A2J4R0U8