Detailed information of TA system TA215708

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41727..41980
• Replicon: plasmid unnamed4
• Accession: NZ_CP082958
• Organism: Salmonella enterica subsp. arizonae strain S499

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K8I78_RS26715
• Protein ID: WP_001312851.1
• Coordinates: 41831..41980 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41727..41786 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8I78_RS26690 (38455)	38455..39201	+	747	WP_000205764.1	conjugal transfer pilus acetylase TraX	-
K8I78_RS26695 (39255)	39255..39815	+	561	WP_001567328.1	fertility inhibition protein FinO	-
K8I78_RS26700 (39947)	39947..40147	+	201	WP_015059022.1	hypothetical protein	-
K8I78_RS26705 (40533)	40533..41132	+	600	WP_032083981.1	PIN domain-containing protein	-
K8I78_RS26710 (41296)	41296..41526	+	231	WP_001736714.1	hypothetical protein	-
- (41727)	41727..41786	-	60	NuclAT_1	-	Antitoxin
- (41727)	41727..41786	-	60	NuclAT_1	-	Antitoxin
- (41727)	41727..41786	-	60	NuclAT_1	-	Antitoxin
- (41727)	41727..41786	-	60	NuclAT_1	-	Antitoxin
K8I78_RS26715 (41831)	41831..41980	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
K8I78_RS26720 (42264)	42264..42512	+	249	WP_000083837.1	replication regulatory protein RepA	-
K8I78_RS26725 (42627)	42627..42805	+	179	Protein_54	protein CopA/IncA	-
K8I78_RS26735 (42824)	42824..43681	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
K8I78_RS26740 (44620)	44620..45273	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
K8I78_RS26745 (45366)	45366..45623	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
K8I78_RS26750 (45556)	45556..45957	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
K8I78_RS26755 (46206)	46206..46621	+	416	Protein_59	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-55	-	1..67319	67319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T215708 WP_001312851.1 NZ_CP082958:41831-41980 [Salmonella enterica subsp. arizonae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T215708 NZ_CP110921:c1380817-1380551 [Methylococcus sp. 16-5]
GTGCGTGTCATCCGTTTCGTTCCGGATGCCGGGGAGGCCTACCTGTACTGGCAGGAGCAGGACAGAAAAACGCTCAAGCG
CATCAATCTTCTGATTACCGCCGCGGCTCGCGAACCGTTTTCAGGTTTGGGAAAGCCGGAACCTTTGCGCGGCAACCTGT
CGGGTTACTGGTCCAGGCGCATCGACGATACTCACCGCCTGGTTTACCGCGCCACGGACGATGATCTGGTGATCATCGCC
TGCCGTTTCCATTACTACGGCTCATAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT215708 NZ_CP082958:c41786-41727 [Salmonella enterica subsp. arizonae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin