Detailed information of TA system TA215673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 53743..54265
• Replicon: plasmid unnamed2
• Accession: NZ_CP082938
• Organism: Klebsiella pneumoniae strain NDM-71

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: K8M01_RS26670
• Protein ID: WP_004181778.1
• Coordinates: 53981..54265 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: K8M01_RS26665
• Protein ID: WP_004181777.1
• Coordinates: 53743..53991 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8M01_RS26645 (K8M01_26580)	48952..49773	-	822	WP_004181772.1	hypothetical protein	-
K8M01_RS26650 (K8M01_26585)	49835..50188	-	354	WP_004181774.1	hypothetical protein	-
K8M01_RS26655 (K8M01_26590)	50333..51319	-	987	WP_025368599.1	hypothetical protein	-
K8M01_RS26660 (K8M01_26595)	51653..53452	-	1800	WP_230796768.1	ATP-dependent helicase	-
K8M01_RS26665 (K8M01_26600)	53743..53991	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K8M01_RS26670 (K8M01_26605)	53981..54265	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8M01_RS26675 (K8M01_26610)	54282..54383	-	102	Protein_58	IS200/IS605 family transposase	-
K8M01_RS26680 (K8M01_26615)	54419..55645	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
K8M01_RS26685 (K8M01_26620)	55916..56050	-	135	Protein_60	zinc ribbon domain-containing protein	-
K8M01_RS26690 (K8M01_26625)	56066..57274	-	1209	WP_263091609.1	IS4-like element ISVsa5 family transposase	-
K8M01_RS26695 (K8M01_26630)	57557..57985	-	429	WP_077257669.1	IS200/IS605 family transposase	-
K8M01_RS26700 (K8M01_26635)	58021..59208	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..299755	299755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T215673 WP_004181778.1 NZ_CP082938:53981-54265 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T215673 NZ_CP110887:2753110-2753604 [Dickeya solani]
ATGATCTCTGCTCCTGAGCCACTCCACGCCGAACATGTACTATCTTCATTCTGTTGCGGTGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAGAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACAACTGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAGGGCGTTGGCCGGGCGCTGGTGCGTGATGC
CGGGCTACGGGTGATTCAGGTAGCGGATACTATCGGCATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAAGCGCGTG
ATTTTTACCTACGAGTGGGGTTTGAACCTTCACCGATTGACCCGATGATTTTGATGGTAACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT215673 WP_004181777.1 NZ_CP082938:53743-53991 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT215673 NZ_CP110887:2752820-2753113 [Dickeya solani]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGATCTGATCGA
TCGTGCAGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTTCTTGAGGCTGCTCGCGCCGCCGCAGAGGAAGCGTTGA
TCGATCAGCGTATCATCATGGCAGATCCAGATGCCTATCAGGAATTCCTTGCTCGCTTGGATCAGGCTCCTGAGCCAAAT
GCCGCGCTGCGTAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8