Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4344168..4344814 Replicon chromosome
Accession NZ_CP082936
Organism Klebsiella pneumoniae strain NDM-71

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag K8M01_RS20970 Protein ID WP_004174016.1
Coordinates 4344168..4344515 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A231WWU8
Locus tag K8M01_RS20975 Protein ID WP_004174017.1
Coordinates 4344515..4344814 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8M01_RS20960 (4340094) 4340094..4341527 + 1434 WP_002920564.1 glycogen synthase GlgA -
K8M01_RS20965 (4341545) 4341545..4343992 + 2448 WP_002920561.1 glycogen phosphorylase -
K8M01_RS20970 (4344168) 4344168..4344515 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K8M01_RS20975 (4344515) 4344515..4344814 + 300 WP_004174017.1 XRE family transcriptional regulator Antitoxin
K8M01_RS20980 (4344877) 4344877..4346385 - 1509 WP_004174018.1 glycerol-3-phosphate dehydrogenase -
K8M01_RS20985 (4346590) 4346590..4346919 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K8M01_RS20990 (4346970) 4346970..4347800 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K8M01_RS20995 (4347850) 4347850..4348608 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T215670 WP_004174016.1 NZ_CP082936:4344168-4344515 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T215670 NZ_CP110886:c4810439-4810035 [Dickeya solani]
ATGAACATACGGATTTTCAAGTCAACGCTGATCCGTCAACAATTGAGCCAGCAAGAGCTTGATGATTTGGTCGCGGATTT
TCACGCCTATAAGCAGAATGGGGTACTACCGGACACATTTGGGCGTGACGCGCCTTATGATGATGACCGAACCTATCCAT
TAGTAAAAGCAGAGCAGGTGGCTCACATCCACCTTGCCGATGCGGATGCGCCTTTTCCCAGGTTCTTGCGCCAGTTCAAG
AGAACCAGCGATCGGGCCCATTTGGTCTACTGCCAGGGAGCGATGGATCCCGAGGCCTGCCTGTTAATTATTATTTTAAA
ACCCGAAGCCCATAAAATGGCACGCAACAATAACAACATGCACAAGATCGGGATGATGGCCGCTGCGTTCAGGATGAAAT
ATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10844.64 Da        Isoelectric Point: 10.1464

>AT215670 WP_004174017.1 NZ_CP082936:4344515-4344814 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT215670 NZ_CP110886:c4810729-4810436 [Dickeya solani]
ATGGAGCGTATTCTTGCTGAAAAATCTATAAATATTACAGAGTTAAGGAAAAATCCGGCTAAGTATTTTATTGATGAGCC
TGTTGCCGTGCTTTCTAACAACCGGCCCGCCGGTTACATGGTGAGTGCCAAAGTCTTTGAAGAACTGATCGATCTGCTAG
AGGCGAAGCAGGAAACGGTTCATACCGTAGCGCGCTTTAGGCCAACGGCCGAACGTTTACGAGCCCTTGCAGATAGCGGT
CAGAAACTGCTGAATGATGCCTCTGGCAAGGATCTGACTGAGTTCACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A231WWU8

References