Detailed information of TA system TA215572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2073777..2074379
• Replicon: chromosome
• Accession: NZ_CP082916
• Organism: Salmonella enterica subsp. enterica serovar Newlands strain ZC-S1 3rd

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M7S4R6
• Locus tag: K8862_RS10080
• Protein ID: WP_001159635.1
• Coordinates: 2074068..2074379 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K8862_RS10075
• Protein ID: WP_000362050.1
• Coordinates: 2073777..2074067 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8862_RS10060 (2071270)	2071270..2072172	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
K8862_RS10065 (2072169)	2072169..2072804	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K8862_RS10070 (2072801)	2072801..2073730	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
K8862_RS10075 (2073777)	2073777..2074067	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
K8862_RS10080 (2074068)	2074068..2074379	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
K8862_RS10085 (2074597)	2074597..2075526	+	930	WP_001127705.1	alpha/beta hydrolase	-
K8862_RS10090 (2075612)	2075612..2075923	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	-
K8862_RS10095 (2075920)	2075920..2076366	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
K8862_RS10100 (2076381)	2076381..2077322	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K8862_RS10105 (2077367)	2077367..2077804	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
K8862_RS10110 (2077801)	2077801..2078673	-	873	WP_000921423.1	virulence factor BrkB family protein	-
K8862_RS10115 (2078667)	2078667..2079266	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12340.30 Da
• Isoelectric Point: 9.4460

>T215572 WP_001159635.1 NZ_CP082916:c2074379-2074068 [Salmonella enterica subsp. enterica serovar Newlands]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T215572 NZ_CP110816:974677-975012 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT215572 WP_000362050.1 NZ_CP082916:c2074067-2073777 [Salmonella enterica subsp. enterica serovar Newlands]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT215572 NZ_CP110816:974429-974677 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A3V2JK97