Detailed information of TA system TA021545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 4644613..4645190
• Replicon: chromosome
• Accession: NC_007951
• Organism: Burkholderia xenovorans LB400

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: BXE_RS20940
• Protein ID: WP_038458473.1
• Coordinates: 4644912..4645190 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: BXE_RS20935
• Protein ID: WP_011490165.1
• Coordinates: 4644613..4644870 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXE_RS20915	4639720..4640472	+	753	WP_011490161.1	5-oxoprolinase subunit PxpA	-
BXE_RS20920	4640750..4641499	+	750	WP_011490162.1	DUF969 domain-containing protein	-
BXE_RS20925	4641496..4642452	+	957	WP_011490163.1	DUF979 domain-containing protein	-
BXE_RS20930	4642668..4644473	+	1806	WP_011490164.1	tetratricopeptide repeat protein	-
BXE_RS20935	4644613..4644870	-	258	WP_011490165.1	BrnA antitoxin family protein	Antitoxin
BXE_RS20940	4644912..4645190	-	279	WP_038458473.1	BrnT family toxin	Toxin
BXE_RS20945	4645388..4646539	-	1152	WP_011490167.1	TraB/GumN family protein	-
BXE_RS20950	4646532..4647551	-	1020	WP_011490168.1	ABC transporter ATP-binding protein	-
BXE_RS20955	4647548..4648552	-	1005	WP_011490169.1	ABC transporter ATP-binding protein	-
BXE_RS20960	4648552..4649469	-	918	WP_011490170.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (24-77)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.17 Da
• Isoelectric Point: 6.4819

>T21545 WP_038458473.1 NC_007951:c4645190-4644912 [Paraburkholderia xenovorans LB400]
VQFEWDEAKNQINIRKHGIDFQDAIDVFNHPVLTALDTREDYGEDRWIALGWMAAVVGVVVYVERSADVIRIISARKATR
HELGHYKQRIWN

Nucleotide

• Length: 279 bp

>T21545 NC_007951:c4645190-4644912 [Paraburkholderia xenovorans LB400]
GTGCAGTTCGAGTGGGACGAAGCCAAAAATCAAATCAATATCCGCAAACACGGCATCGACTTTCAGGATGCCATCGACGT
GTTCAATCATCCCGTACTTACGGCACTAGACACGCGGGAAGATTACGGCGAGGACCGGTGGATCGCATTGGGCTGGATGG
CGGCTGTCGTGGGCGTGGTGGTGTACGTGGAGCGCAGTGCGGATGTGATCCGGATCATTTCGGCCCGCAAGGCAACCAGG
CACGAACTCGGACATTACAAGCAGCGCATCTGGAATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9943.28 Da
• Isoelectric Point: 9.6907

>AT21545 WP_011490165.1 NC_007951:c4644870-4644613 [Paraburkholderia xenovorans LB400]
MGKTSGTDWKRLARTDDAQIDKSDVPELGDEFFERAELHVPPKQAVTMRLDADVLNWFKEQGQGYQTRINKLLRAYMLAH
QRRRP

Nucleotide

• Length: 258 bp

>AT21545 NC_007951:c4644870-4644613 [Paraburkholderia xenovorans LB400]
ATGGGCAAAACATCGGGAACGGATTGGAAGCGACTCGCCAGAACGGACGATGCGCAAATCGATAAGAGCGACGTACCTGA
GTTGGGCGACGAGTTTTTTGAGCGCGCCGAACTGCATGTTCCTCCAAAGCAGGCCGTGACAATGCGTCTGGATGCGGACG
TGTTGAACTGGTTCAAGGAGCAAGGTCAAGGCTATCAGACGCGGATCAACAAATTGTTGCGCGCCTATATGCTGGCACAT
CAGCGCCGGCGTCCTTGA