Detailed information of TA system TA215262

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12480..12744
• Replicon: plasmid unnamed2
• Accession: NZ_CP082826
• Organism: Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K8B79_RS25925
• Protein ID: WP_001387489.1
• Coordinates: 12480..12632 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	12684..12744 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B79_RS25895 (7746)	7746..9914	+	2169	WP_015508354.1	DotA/TraY family protein	-
K8B79_RS25900 (9985)	9985..10647	+	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
K8B79_RS25905 (10719)	10719..10928	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K8B79_RS25910 (11320)	11320..11496	+	177	WP_001054900.1	hypothetical protein	-
K8B79_RS25915 (11561)	11561..11857	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K8B79_RS25920 (12157)	12157..12408	+	252	WP_001291964.1	hypothetical protein	-
K8B79_RS25925 (12480)	12480..12632	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (12684)	12684..12744	+	61	NuclAT_0	-	Antitoxin
- (12684)	12684..12744	+	61	NuclAT_0	-	Antitoxin
- (12684)	12684..12744	+	61	NuclAT_0	-	Antitoxin
- (12684)	12684..12744	+	61	NuclAT_0	-	Antitoxin
K8B79_RS25930 (12953)	12953..14038	+	1086	WP_000080543.1	protein finQ	-
- (14108)	14108..14165	+	58	NuclAT_1	-	-
- (14108)	14108..14165	+	58	NuclAT_1	-	-
- (14108)	14108..14165	+	58	NuclAT_1	-	-
- (14108)	14108..14165	+	58	NuclAT_1	-	-
K8B79_RS25935 (14345)	14345..15553	+	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
K8B79_RS25940 (15572)	15572..16642	+	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..86164	86164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T215262 WP_001387489.1 NZ_CP082826:c12632-12480 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T215262 NZ_CP110619:c1947633-1947244 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT215262 NZ_CP082826:12684-12744 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin