Detailed information of TA system TA215225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5006324..5006910
• Replicon: chromosome
• Accession: NZ_CP082823
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: K8B71_RS24085
• Protein ID: WP_003120987.1
• Coordinates: 5006611..5006910 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K8B71_RS24080
• Protein ID: WP_003448662.1
• Coordinates: 5006324..5006614 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B71_RS24060 (K8B71_24060)	5001475..5001684	+	210	WP_003105733.1	cold-shock protein	-
K8B71_RS24065 (K8B71_24065)	5001906..5003795	+	1890	WP_016851610.1	hypothetical protein	-
K8B71_RS24070 (K8B71_24070)	5003792..5005768	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
K8B71_RS24075 (K8B71_24075)	5005909..5006253	+	345	WP_016851612.1	hypothetical protein	-
K8B71_RS24080 (K8B71_24080)	5006324..5006614	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
K8B71_RS24085 (K8B71_24085)	5006611..5006910	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B71_RS24090 (K8B71_24090)	5007112..5008236	+	1125	WP_004347965.1	TcpQ domain-containing protein	-
K8B71_RS24095 (K8B71_24095)	5008236..5009945	+	1710	WP_004347964.1	PilN family type IVB pilus formation outer membrane protein	-
K8B71_RS24100 (K8B71_24100)	5009949..5011274	+	1326	WP_004347963.1	type 4b pilus protein PilO2	-
K8B71_RS24105 (K8B71_24105)	5011264..5011797	+	534	WP_004347962.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4979343..5062091	82748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T215225 WP_003120987.1 NZ_CP082823:c5006910-5006611 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T215225 NZ_CP110535:4572328-4572792 [Halomonas sp. BDJS001]
ATGAGCGAGGTCACCGCTTACCGGCTGGTGAAGCGCAAGTTTCAGGAGACAGCGTTTGATGGCGAAGGCGCACGGCTTTA
CGGTGGGCGCTGGAACAGCCCAGGCAATGCCTGTGTGTATGTGGCAAGCACTGAATCGCTGGCGCTGCTGGAAATCATGG
TGCCTCTGGAGAGCTATCGGCTGCTCAACGCCTATGCGCTACTGCGCCTGACGCTGCCCGCCGAAAGCATTCTCAGCGTT
GGCGCCGAAGATTTACCTGCAAACTGGCGGGAAGCACCCGTGCCCGCAGAGACCGCCGAACTAGGTGATGGCTGGCTAGC
CTCCAACCAGAGTCTGGCACTGGCAGTGCCAAGCGTGGTCGTGCCGAGAGAGCTCAATTACATGCTCAACCCGGCACACC
CCTTATTCGATCAAATCATCGCAGCGGCCGAGGCACTGCCCTTCCAACCCGACCCAAGACTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT215225 WP_003448662.1 NZ_CP082823:c5006614-5006324 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT215225 NZ_CP110535:4571882-4572331 [Halomonas sp. BDJS001]
ATGGATGTCCATTTCACTCAGTACCAACCACCCAAAGCCTTGGCGTTCGGTTTCTGGCAACAAATTGGCCTGCCTGCCCG
TGGCCGAAAGCTGCATGAGTTATTGCATGAAGGCGTTGAGTACCGGGTTTACCCTCAGCTTGCCAAAGTGTCAGGTATTG
AGCAGAAAGCACTGGCGAGCTATGTGGACATTCCCCCTGCCACGTTGAGCCGTCGCGCCAAATCGGGCCGTTTCAAGATG
GCGGAAAGCGACCGGCTGTATCGCTTTGCTGAACTCTTTAAAGCGGCGCTGGACCTGTTTGAAGGCGACGCCGATGGCGC
ACGCGCTTGGCTGCTCAAGCCTAATGCAGGGCTAGGCGGGCGCCGTCCTGTCGAAATGAGCGCAACCTCGGCGGAGTATC
AAACGGTGCTTAACTTGATCGGGCGGCTTGAGCATGGTGTCTCGGTATGA

Structures

Toxin

• AlphaFold DB: G8CP73