Detailed information of TA system TA215220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 151160..151665
• Replicon: chromosome
• Accession: NZ_CP082823
• Organism: Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: K8B71_RS00685
• Protein ID: WP_003083773.1
• Coordinates: 151160..151441 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: K8B71_RS00690
• Protein ID: WP_003083775.1
• Coordinates: 151438..151665 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B71_RS00660 (K8B71_00660)	146411..147760	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
K8B71_RS00665 (K8B71_00665)	147809..148495	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
K8B71_RS00670 (K8B71_00670)	148596..149330	+	735	WP_003128333.1	GntR family transcriptional regulator	-
K8B71_RS00675 (K8B71_00675)	149510..149920	+	411	WP_003110659.1	aegerolysin family protein	-
K8B71_RS00680 (K8B71_00680)	149952..150860	-	909	WP_003083769.1	LysR family transcriptional regulator	-
K8B71_RS00685 (K8B71_00685)	151160..151441	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
K8B71_RS00690 (K8B71_00690)	151438..151665	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
K8B71_RS00695 (K8B71_00695)	151841..152461	-	621	WP_003101226.1	hypothetical protein	-
K8B71_RS00700 (K8B71_00700)	152562..153062	+	501	WP_003128336.1	LEA type 2 family protein	-
K8B71_RS00705 (K8B71_00705)	153135..153476	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
K8B71_RS00710 (K8B71_00710)	153558..154985	-	1428	WP_003083784.1	GABA permease	-
K8B71_RS00715 (K8B71_00715)	155154..156647	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T215220 WP_003083773.1 NZ_CP082823:c151441-151160 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T215220 NZ_CP110535:c587173-586904 [Halomonas sp. BDJS001]
ATGTATAGCCTCGAATACTCAACGCAGTTCAAGAAAGACTTCAAAAAAGTCACCAAAATGCCCATTGCTGACATACTGGA
AGTCGGAAACATTATTTCCCAGCTTCAGCGCGGCGAGCCATTGCATCCTAAGCATGTAGACCACGCGCTAACAGGAAACT
GGTATGGGTTCCGCGATTGCCATATCAAACCAGATCTCGTACTCATCTACAGAGTCGCAAATAAAAATCTGCAACTCGCA
AGAATCGGATCTCATAGCGATCTTTTCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT215220 WP_003083775.1 NZ_CP082823:c151665-151438 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT215220 NZ_CP110535:c587444-587166 [Halomonas sp. BDJS001]
ATGAAAAGCGAAATGCTCAGCACACGCATTGATCATGATACAAAAGTTGCCTTCACTACTATCTGCGATGAGGTTGGCCT
GAGTCCCTCTCAAGCGCTCAAGCTATTTGCACGTGCAGTCATCAACCATGGCGGCATCCCCTTCGAGCTGAAAGCTCGAC
AGCCCAATGAAACAACCTCAGCCGCTATTGATGAGCTCATCCAGGGTAAAGGACACAAAGCCGCTTCGGTTAACACGATG
ATGGATGAATTAACCGAAGCCAACGTAAGCAATGTATAG

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9