Detailed information of TA system TA215200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1613974..1614642
• Replicon: chromosome
• Accession: NZ_CP082820
• Organism: Streptococcus pneumoniae strain SCAID PHRX1-2021

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K8B76_RS07935
• Protein ID: WP_001132283.1
• Coordinates: 1614463..1614642 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: K8B76_RS07930
• Protein ID: WP_000961810.1
• Coordinates: 1613974..1614426 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B76_RS07900 (K8B76_07900)	1609828..1610571	-	744	WP_001188213.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
K8B76_RS07905 (K8B76_07905)	1610573..1611526	-	954	WP_000970781.1	50S ribosomal protein L11 methyltransferase	-
K8B76_RS07910 (K8B76_07910)	1611661..1612134	-	474	WP_000631889.1	GNAT family N-acetyltransferase	-
K8B76_RS07915 (K8B76_07915)	1612131..1612565	-	435	WP_000421871.1	NUDIX hydrolase	-
K8B76_RS07920 (K8B76_07920)	1612615..1613157	-	543	WP_000891117.1	hypothetical protein	-
K8B76_RS07925 (K8B76_07925)	1613213..1613683	-	471	WP_000257097.1	DUF3013 family protein	-
K8B76_RS07930 (K8B76_07930)	1613974..1614426	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K8B76_RS07935 (K8B76_07935)	1614463..1614642	-	180	WP_001132283.1	type II toxin-antitoxin system HicA family toxin	Toxin
K8B76_RS07940 (K8B76_07940)	1614779..1614958	-	180	WP_001809903.1	hypothetical protein	-
K8B76_RS07945 (K8B76_07945)	1615123..1615362	-	240	WP_000818079.1	hypothetical protein	-
K8B76_RS07950 (K8B76_07950)	1615632..1616903	+	1272	WP_001113216.1	replication-associated recombination protein A	-
K8B76_RS07960 (K8B76_07960)	1617323..1618450	-	1128	WP_000323402.1	tyrosine-type recombinase/integrase	-
K8B76_RS07965 (K8B76_07965)	1618452..1618634	-	183	WP_000785802.1	DUF3173 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6596.78 Da
• Isoelectric Point: 10.8214

>T215200 WP_001132283.1 NZ_CP082820:c1614642-1614463 [Streptococcus pneumoniae]
IPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELNKYTERGIGKQAGL

Nucleotide

• Length: 180 bp

>T215200 NZ_CP110533:c78085-77735 [Enterobacter quasiroggenkampii]
ATGTGGACGGTTCGGTTCGGAAAGGTATTCGAGCAGTGGTTGTTTGAACAAGAAGAAGGATTACAGGATAAGGTACTGGC
AGACCTGCTAAATCTGCAACACTATGGGCCACGTCTTCCTCGTCCCTATGCAGATACGGTTAAAGGCTCACGGTATAAGC
ATATGAAAAAATTACGTATTCAATATGCTGGTCGTCCTGTTCCTACTTTCTTCGCATTTGATCCTGTACGTCAGGCCATT
GTGCTTTGCGCGGGCGATAAGAGTAACGACAAAACCTTTTATGAAAAAATGATTCGCATCGCAGATGCCGAATTTTCCCT
TCATCTGACAAGCCAAGAGGCAGCCAAGTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT215200 WP_000961810.1 NZ_CP082820:c1614426-1613974 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT215200 NZ_CP110533:c77738-77439 [Enterobacter quasiroggenkampii]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGTTCACCGGAAAGCCAACAACGTATTAAAGAGATGGCAGAGGAAATGAT
CCTCGAAACGGGTCTGCAACTTTTACGTGAAGAGCTCAGGCTTTCGCAAAAAAAAGTTGCCGACCTGATGGGGGTGAGCC
AGCCCGCAGTAACTCAGATTGAGCAACGTGGAAATGAACTAAAAATTGCCACGCTTAAGCGTTACATCGAGGCAATGGGT
GGGAAACTCTGTATCGACGTTGAACTTCCCACAGGAAAACGCGTTGCATTTCATATCTAA

Structures

Antitoxin

• PDB: 5YRZ
• AlphaFold DB: G0IC64