Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2032892..2033511 Replicon chromosome
Accession NZ_CP082791
Organism Klebsiella pneumoniae strain KP4823

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KCV57_RS09855 Protein ID WP_002892050.1
Coordinates 2033293..2033511 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KCV57_RS09850 Protein ID WP_002892066.1
Coordinates 2032892..2033266 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KCV57_RS09840 (2028044) 2028044..2029237 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KCV57_RS09845 (2029260) 2029260..2032406 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KCV57_RS09850 (2032892) 2032892..2033266 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KCV57_RS09855 (2033293) 2033293..2033511 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KCV57_RS09860 (2033670) 2033670..2034236 + 567 WP_002892030.1 maltose O-acetyltransferase -
KCV57_RS09865 (2034208) 2034208..2034348 - 141 WP_004147370.1 hypothetical protein -
KCV57_RS09870 (2034369) 2034369..2034839 + 471 WP_002892026.1 YlaC family protein -
KCV57_RS09875 (2034814) 2034814..2036265 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KCV57_RS09880 (2036366) 2036366..2037064 + 699 WP_002892021.1 GNAT family protein -
KCV57_RS09885 (2037061) 2037061..2037201 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KCV57_RS09890 (2037201) 2037201..2037464 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215077 WP_002892050.1 NZ_CP082791:2033293-2033511 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215077 NZ_CP110398:c2617710-2617420 [Shigella sonnei]
ATGTTACCCGTTTTATGGCTACCATCTGCTCGTGATGATTTGCGCCAGATCGTAGCCTACATAGCTAAAGAAAATCCCGC
GGCAGCACGCAGAATGAAAATTCGAATTGAAACATCCGTTTTGCCGCTCACAGAGCATCCTTACCTGTATCCTCCAAGCG
AGCGAGTTCTTGGTCTTCGTGAAATTGTAACTCATCCTAACTACATCATCCTTTACCGAGTAACAGCATCAAGCATAGAA
GTAGTAAATATTGTGCATTCTCGAAGACAGTATCCAGGCAAAAACAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215077 WP_002892066.1 NZ_CP082791:2032892-2033266 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215077 NZ_CP110398:c2617904-2617710 [Shigella sonnei]
ATGAGTACAGTCCTTTCCCCAATGGTTTCAGAATTTGAGACAGTTGAGCAAGAAAATAGTTACAACGAATGGCTGCGGGC
CAAAGTAGCAGCAAGCCTCGCGGATCCCCGCCCGGCGATTCCGCATGATGAGGTAATGGCGGAAATGGAACAAATTATTG
CCCAAATTGCTGCTGCAACTCACAGAGGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References