Detailed information of TA system TA215016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4446887..4447686
• Replicon: chromosome
• Accession: NZ_CP082777
• Organism: Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: K8B91_RS21310
• Protein ID: WP_000347252.1
• Coordinates: 4447222..4447686 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: K8B91_RS21305
• Protein ID: WP_001296435.1
• Coordinates: 4446887..4447222 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B91_RS21290 (4442672)	4442672..4443442	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
K8B91_RS21295 (4443458)	4443458..4444792	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
K8B91_RS21300 (4445167)	4445167..4446738	+	1572	WP_001273940.1	galactarate dehydratase	-
K8B91_RS21305 (4446887)	4446887..4447222	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K8B91_RS21310 (4447222)	4447222..4447686	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K8B91_RS21315 (4447741)	4447741..4448550	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
K8B91_RS21320 (4448799)	4448799..4450079	+	1281	WP_000681925.1	tagatose-bisphosphate aldolase subunit KbaZ	-
K8B91_RS21325 (4450102)	4450102..4450575	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K8B91_RS21330 (4450586)	4450586..4451365	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
K8B91_RS21335 (4451355)	4451355..4452233	+	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
K8B91_RS21340 (4452251)	4452251..4452685	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T215016 WP_000347252.1 NZ_CP082777:4447222-4447686 [Escherichia coli O25:H1]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T215016 NZ_CP110369:780885-781295 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT215016 WP_001296435.1 NZ_CP082777:4446887-4447222 [Escherichia coli O25:H1]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT215016 NZ_CP110369:780638-780904 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5