Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 174968..175570 Replicon chromosome
Accession NZ_CP082777
Organism Escherichia coli O25:H1 strain CU39ES-1

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag K8B91_RS00840 Protein ID WP_000897302.1
Coordinates 174968..175279 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K8B91_RS00845 Protein ID WP_000356397.1
Coordinates 175280..175570 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B91_RS00815 (170881) 170881..171480 + 600 WP_001443191.1 glucose-1-phosphatase -
K8B91_RS00820 (171474) 171474..172346 + 873 WP_000920762.1 virulence factor BrkB family protein -
K8B91_RS00825 (172343) 172343..172780 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
K8B91_RS00830 (172825) 172825..173766 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
K8B91_RS00835 (173830) 173830..174738 - 909 WP_001305059.1 alpha/beta hydrolase -
K8B91_RS00840 (174968) 174968..175279 + 312 WP_000897302.1 hypothetical protein Toxin
K8B91_RS00845 (175280) 175280..175570 + 291 WP_000356397.1 NadS family protein Antitoxin
K8B91_RS00850 (175929) 175929..176207 + 279 WP_001296612.1 hypothetical protein -
K8B91_RS00855 (176603) 176603..176821 + 219 WP_001314326.1 CopG family transcriptional regulator -
K8B91_RS00860 (177037) 177037..177966 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
K8B91_RS00865 (177963) 177963..178598 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
K8B91_RS00870 (178595) 178595..179497 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T214990 WP_000897302.1 NZ_CP082777:174968-175279 [Escherichia coli O25:H1]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T214990 NZ_CP110354:c2770548-2770445 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT214990 WP_000356397.1 NZ_CP082777:175280-175570 [Escherichia coli O25:H1]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT214990 NZ_CP110354:2770432-2770641 [Enterobacter hormaechei]
TTTTCAAGCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGCA
AAAGTGGTCTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References