Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 2337652..2339240 | Replicon | chromosome |
| Accession | NZ_CP082776 | ||
| Organism | Escherichia coli O4:H5 strain CU532-9 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | B7MMY1 |
| Locus tag | K8B92_RS11430 | Protein ID | WP_001125475.1 |
| Coordinates | 2337652..2338974 (-) | Length | 441 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | - |
| Locus tag | K8B92_RS11435 | Protein ID | WP_001332145.1 |
| Coordinates | 2338974..2339240 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K8B92_RS11390 (2333291) | 2333291..2333524 | - | 234 | WP_000473544.1 | hypothetical protein | - |
| K8B92_RS11395 (2333559) | 2333559..2333810 | - | 252 | WP_001536107.1 | hypothetical protein | - |
| K8B92_RS11400 (2333813) | 2333813..2334130 | - | 318 | WP_000073835.1 | DNA-binding protein | - |
| K8B92_RS11405 (2334142) | 2334142..2335194 | - | 1053 | WP_000568107.1 | phage/plasmid replication protein, II/X family | - |
| K8B92_RS11410 (2335366) | 2335366..2335812 | + | 447 | WP_000527682.1 | helix-turn-helix transcriptional regulator | - |
| K8B92_RS11415 (2335912) | 2335912..2336334 | + | 423 | WP_001527022.1 | hypothetical protein | - |
| K8B92_RS11420 (2336402) | 2336402..2337196 | + | 795 | WP_001527023.1 | hypothetical protein | - |
| K8B92_RS11425 (2337207) | 2337207..2337473 | + | 267 | WP_001527024.1 | hypothetical protein | - |
| K8B92_RS11430 (2337652) | 2337652..2338974 | - | 1323 | WP_001125475.1 | type II toxin-antitoxin system serine/threonine protein kinase toxin HipA | Toxin |
| K8B92_RS11435 (2338974) | 2338974..2339240 | - | 267 | WP_001332145.1 | type II toxin-antitoxin system antitoxin HipB | Antitoxin |
| K8B92_RS11440 (2339548) | 2339548..2340306 | + | 759 | WP_001286588.1 | trans-aconitate 2-methyltransferase | - |
| K8B92_RS11445 (2340310) | 2340310..2341224 | - | 915 | WP_001306069.1 | bestrophin family protein | - |
| K8B92_RS11450 (2341421) | 2341421..2342872 | - | 1452 | WP_000854636.1 | tagaturonate reductase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | focC | 2300605..2339240 | 38635 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 441 a.a. Molecular weight: 49426.24 Da Isoelectric Point: 8.9380
>T214974 WP_001125475.1 NZ_CP082776:c2338974-2337652 [Escherichia coli O4:H5]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK
Download Length: 1323 bp
>T214974 NZ_CP110352:c147772-147491 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACTTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACTTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 10016.44 Da Isoelectric Point: 6.2267
>AT214974 WP_001332145.1 NZ_CP082776:c2339240-2338974 [Escherichia coli O4:H5]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW
Download Length: 267 bp
>AT214974 NZ_CP110352:c147996-147769 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|