Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4381199..4381998 Replicon chromosome
Accession NZ_CP082775
Organism Escherichia coli O25:H4 strain CU568-3

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag K8B89_RS21660 Protein ID WP_000347251.1
Coordinates 4381534..4381998 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag K8B89_RS21655 Protein ID WP_001296435.1
Coordinates 4381199..4381534 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B89_RS21640 (4376984) 4376984..4377754 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
K8B89_RS21645 (4377770) 4377770..4379104 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
K8B89_RS21650 (4379479) 4379479..4381050 + 1572 WP_001273763.1 galactarate dehydratase -
K8B89_RS21655 (4381199) 4381199..4381534 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K8B89_RS21660 (4381534) 4381534..4381998 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K8B89_RS21665 (4382053) 4382053..4382862 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K8B89_RS21670 (4383111) 4383111..4384391 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
K8B89_RS21675 (4384414) 4384414..4384887 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K8B89_RS21680 (4384898) 4384898..4385677 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K8B89_RS21685 (4385667) 4385667..4386545 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K8B89_RS21690 (4386563) 4386563..4386997 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T214966 WP_000347251.1 NZ_CP082775:4381534-4381998 [Escherichia coli O25:H4]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T214966 NZ_CP110350:c5932375-5931620 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT214966 WP_001296435.1 NZ_CP082775:4381199-4381534 [Escherichia coli O25:H4]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT214966 NZ_CP110350:c5932878-5932510 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References