Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4037357..4038011 Replicon chromosome
Accession NZ_CP082774
Organism Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag K8B88_RS19715 Protein ID WP_000244781.1
Coordinates 4037357..4037764 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K8B88_RS19720 Protein ID WP_000354046.1
Coordinates 4037745..4038011 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B88_RS19695 (4033314) 4033314..4035047 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
K8B88_RS19700 (4035053) 4035053..4035763 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K8B88_RS19705 (4035788) 4035788..4036684 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K8B88_RS19710 (4036796) 4036796..4037317 + 522 WP_001055874.1 flavodoxin FldB -
K8B88_RS19715 (4037357) 4037357..4037764 - 408 WP_000244781.1 protein YgfX Toxin
K8B88_RS19720 (4037745) 4037745..4038011 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K8B88_RS19725 (4038254) 4038254..4039234 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
K8B88_RS19730 (4039311) 4039311..4039970 - 660 WP_000250274.1 hemolysin III family protein -
K8B88_RS19735 (4040134) 4040134..4040445 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
K8B88_RS19740 (4040490) 4040490..4041923 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T214940 WP_000244781.1 NZ_CP082774:c4037764-4037357 [Escherichia coli O16:H6]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214940 NZ_CP110346:c5119393-5119046 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCTGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214940 WP_000354046.1 NZ_CP082774:c4038011-4037745 [Escherichia coli O16:H6]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214940 NZ_CP110346:c5119654-5119403 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References