Detailed information of TA system TA214918

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 826286..827118
• Replicon: chromosome
• Accession: NZ_CP082774
• Organism: Escherichia coli O16:H6 strain CU37RT-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K8B88_RS03935
• Protein ID: WP_000854754.1
• Coordinates: 826744..827118 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: K8B88_RS03930
• Protein ID: WP_001315620.1
• Coordinates: 826286..826654 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B88_RS03900 (823115)	823115..823570	+	456	WP_000581506.1	IrmA family protein	-
K8B88_RS03905 (823671)	823671..823879	+	209	Protein_750	DUF905 family protein	-
K8B88_RS03910 (823980)	823980..824801	+	822	WP_021538251.1	DUF932 domain-containing protein	-
K8B88_RS03915 (824856)	824856..825341	+	486	WP_021538252.1	antirestriction protein	-
K8B88_RS03920 (825357)	825357..825833	+	477	WP_001424026.1	RadC family protein	-
K8B88_RS03925 (825902)	825902..826123	+	222	WP_000692345.1	DUF987 domain-containing protein	-
K8B88_RS03930 (826286)	826286..826654	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B88_RS03935 (826744)	826744..827118	+	375	WP_000854754.1	TA system toxin CbtA family protein	Toxin
K8B88_RS03940 (827115)	827115..827603	+	489	WP_021538253.1	DUF5983 family protein	-
K8B88_RS03945 (827615)	827615..827812	+	198	WP_000839291.1	DUF957 domain-containing protein	-
K8B88_RS03950 (827897)	827897..828046	+	150	Protein_759	restriction endonuclease subunit M	-
K8B88_RS03955 (828814)	828814..828957	-	144	Protein_760	HNH endonuclease	-
K8B88_RS03960 (829094)	829094..829744	+	651	WP_001037966.1	HNH endonuclease	-
K8B88_RS03965 (829752)	829752..830732	-	981	WP_001295601.1	sialate O-acetylesterase	-
K8B88_RS03970 (830797)	830797..831903	-	1107	WP_001315214.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14019.97 Da
• Isoelectric Point: 8.2905

>T214918 WP_000854754.1 NZ_CP082774:826744-827118 [Escherichia coli O16:H6]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEKR

Nucleotide

• Length: 375 bp

>T214918 NZ_CP110344:c2605486-2605163 [Pseudomonas aeruginosa]
CTGTCACCCTTTGAGCGCAACCGAAAAGCCCACCTCGATGATGACGAGTACTCGCTATTCCAGCAGATGCTCTTGACCAA
CCCGGAGGCAGGCGCAGTCATCGCCAACAGCGGTGGTCTGAGGAAAGTCCGGTTCGGATCGGTCAGGCGCAGCAAGGGAA
AGCGAGGTGGCGTTCGAGTGATTTATTACTACTGGCACAGTGGTCTGCAGTTCTGGCTCTTCACCCTGTATGACAAAGAC
GAACTCGATGACCTGAGCAGCGACCAGCGTAGGCAGTTGAAAGCGCTCCTTGAGCGCGAAGTGAAGGCGAGGCAAACACC
ATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT214918 WP_001315620.1 NZ_CP082774:826286-826654 [Escherichia coli O16:H6]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT214918 NZ_CP110344:c2605166-2604858 [Pseudomonas aeruginosa]
ATGACCAAACGCGATATTTTTTCCGAGCTGATCGAAGGCTTCGACGACCTGGCCTCGGCACGCGAAGGCAAGCGTACGCT
GCGTACTCACAAGGTTGAATACAAGCCAATTGAACCCGCGACAGGCGCGGAAATCGTCGCACTGCGCGAAAAGCTGCAGA
TGTCCCAGGGTGTATTTGCGCAGGCCATTCACGCCAAGCCTGCGACCTTAAAGAACTGGGAGCAGAACCGCTCAAAGCCC
AACGACCAGGCTACTGTTCTCATCCGTATCCTGAACAAGCATCCGGACCTCATACGCGAACTGGCGTAG

Structures

Antitoxin

• AlphaFold DB: V0ULY5