Detailed information of TA system TA214850

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3842556..3843210
• Replicon: chromosome
• Accession: NZ_CP082771
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: K8B90_RS18545
• Protein ID: WP_000244781.1
• Coordinates: 3842556..3842963 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: K8B90_RS18550
• Protein ID: WP_000354046.1
• Coordinates: 3842944..3843210 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B90_RS18525 (3838513)	3838513..3840246	-	1734	WP_000813193.1	single-stranded-DNA-specific exonuclease RecJ	-
K8B90_RS18530 (3840252)	3840252..3840962	-	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K8B90_RS18535 (3840987)	3840987..3841883	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
K8B90_RS18540 (3841995)	3841995..3842516	+	522	WP_001055874.1	flavodoxin FldB	-
K8B90_RS18545 (3842556)	3842556..3842963	-	408	WP_000244781.1	protein YgfX	Toxin
K8B90_RS18550 (3842944)	3842944..3843210	-	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
K8B90_RS18555 (3843453)	3843453..3844433	+	981	WP_000886078.1	tRNA-modifying protein YgfZ	-
K8B90_RS18560 (3844510)	3844510..3845169	-	660	WP_000250274.1	hemolysin III family protein	-
K8B90_RS18565 (3845333)	3845333..3845644	-	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
K8B90_RS18570 (3845689)	3845689..3847122	+	1434	WP_001350137.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T214850 WP_000244781.1 NZ_CP082771:c3842963-3842556 [Escherichia coli LF82]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T214850 NZ_CP110287:3490053-3490790 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT214850 WP_000354046.1 NZ_CP082771:c3843210-3842944 [Escherichia coli LF82]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT214850 NZ_CP110287:3490903-3491028 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: S1PAM6

Antitoxin

• PDB: 6B58
• PDB: 1X6I
• PDB: 1X6J
• PDB: 6C12
• AlphaFold DB: A0A7U9QD57