Detailed information of TA system TA214725

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1375696..1376468
• Replicon: chromosome
• Accession: NZ_CP082360
• Organism: Klebsiella sp. CTHL.F3a

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K7H21_RS06585
• Protein ID: WP_032938032.1
• Coordinates: 1375696..1376070 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K7H21_RS06590
• Protein ID: WP_049101822.1
• Coordinates: 1376109..1376468 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7H21_RS06555 (K7H21_06555)	1371378..1371611	-	234	WP_004132711.1	hypothetical protein	-
K7H21_RS06565 (K7H21_06565)	1372011..1372283	+	273	WP_016247220.1	hypothetical protein	-
K7H21_RS06570 (K7H21_06570)	1372486..1373394	+	909	WP_032938037.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-
K7H21_RS06575 (K7H21_06575)	1374168..1375157	+	990	WP_047058004.1	glycosyltransferase	-
K7H21_RS06580 (K7H21_06580)	1375161..1375616	+	456	WP_223226960.1	hypothetical protein	-
K7H21_RS06585 (K7H21_06585)	1375696..1376070	-	375	WP_032938032.1	TA system toxin CbtA family protein	Toxin
K7H21_RS06590 (K7H21_06590)	1376109..1376468	-	360	WP_049101822.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K7H21_RS06595 (K7H21_06595)	1376491..1376712	-	222	WP_032938028.1	DUF987 domain-containing protein	-
K7H21_RS06600 (K7H21_06600)	1376726..1377208	-	483	WP_032938026.1	DNA repair protein RadC	-
K7H21_RS06605 (K7H21_06605)	1377449..1377928	-	480	WP_075206252.1	antirestriction protein	-
K7H21_RS06610 (K7H21_06610)	1378008..1378829	-	822	WP_071067384.1	DUF932 domain-containing protein	-
K7H21_RS06615 (K7H21_06615)	1378940..1379152	-	213	WP_032938024.1	hypothetical protein	-
K7H21_RS06620 (K7H21_06620)	1379262..1379735	-	474	WP_189095326.1	hypothetical protein	-
K7H21_RS06625 (K7H21_06625)	1379809..1380438	-	630	WP_032938019.1	hypothetical protein	-
K7H21_RS06630 (K7H21_06630)	1380536..1381213	-	678	WP_032938016.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1372486..1397683	25197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (9-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13903.92 Da
• Isoelectric Point: 9.7700

>T214725 WP_032938032.1 NZ_CP082360:c1376070-1375696 [Klebsiella sp. CTHL.F3a]
MQIQSLPPQRAASSRLSSVKIWQKLLEYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGF
SVKEQSPFIGSLDMLRDRKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 375 bp

>T214725 NZ_CP110198:2446154-2446438 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13393.59 Da
• Isoelectric Point: 8.8636

>AT214725 WP_049101822.1 NZ_CP082360:c1376468-1376109 [Klebsiella sp. CTHL.F3a]
MQKATRVINHNIAEPWWGLRRNVTPCFGARLVQEGNRLHYLADRASTTGTFNDADLRHLDQAFPLLMKQMELMLTSSELM
PRIQRCITLHAKGLICKADTLGSCGYLYIVIYPASATTA

Nucleotide

• Length: 360 bp

>AT214725 NZ_CP110198:2445916-2446164 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA