Detailed information of TA system TA214709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3499600..3500437
• Replicon: chromosome
• Accession: NZ_CP082357
• Organism: Escherichia coli strain VCSW7

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: K7X31_RS16590
• Protein ID: WP_000227784.1
• Coordinates: 3499895..3500437 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: K7X31_RS16585
• Protein ID: WP_001297137.1
• Coordinates: 3499600..3499911 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7X31_RS16560 (3494620)	3494620..3495567	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
K7X31_RS16565 (3495589)	3495589..3497580	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
K7X31_RS16570 (3497570)	3497570..3498184	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
K7X31_RS16575 (3498184)	3498184..3498513	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
K7X31_RS16580 (3498525)	3498525..3499415	+	891	WP_000971339.1	heme o synthase	-
K7X31_RS16585 (3499600)	3499600..3499911	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
K7X31_RS16590 (3499895)	3499895..3500437	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
K7X31_RS16595 (3500493)	3500493..3501428	-	936	WP_024180261.1	tetratricopeptide repeat protein	-
K7X31_RS16600 (3501835)	3501835..3503199	+	1365	WP_223024580.1	MFS transporter	-
K7X31_RS16605 (3503327)	3503327..3503818	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
K7X31_RS16610 (3503986)	3503986..3504897	+	912	WP_000705836.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T214709 WP_000227784.1 NZ_CP082357:3499895-3500437 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T214709 NZ_CP110193:c4676042-4675509 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT214709 WP_001297137.1 NZ_CP082357:3499600-3499911 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT214709 NZ_CP110193:c4676319-4676053 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3