Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 898053..898707 Replicon chromosome
Accession NZ_CP082349
Organism Escherichia coli strain AL.H8

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag K7H97_RS04395 Protein ID WP_000244772.1
Coordinates 898300..898707 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K7H97_RS04390 Protein ID WP_000354046.1
Coordinates 898053..898319 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7H97_RS04365 (893341) 893341..894084 + 744 WP_000951951.1 SDR family oxidoreductase -
K7H97_RS04370 (894141) 894141..895574 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
K7H97_RS04375 (895619) 895619..895930 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
K7H97_RS04380 (896094) 896094..896753 + 660 WP_000250274.1 hemolysin III family protein -
K7H97_RS04385 (896830) 896830..897810 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K7H97_RS04390 (898053) 898053..898319 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K7H97_RS04395 (898300) 898300..898707 + 408 WP_000244772.1 protein YgfX Toxin
K7H97_RS04400 (898747) 898747..899268 - 522 WP_001055874.1 flavodoxin FldB -
K7H97_RS04405 (899380) 899380..900276 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K7H97_RS04410 (900301) 900301..901011 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K7H97_RS04415 (901017) 901017..902750 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T214638 WP_000244772.1 NZ_CP082349:898300-898707 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214638 NZ_CP110160:337422-337790 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214638 WP_000354046.1 NZ_CP082349:898053-898319 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214638 NZ_CP110160:337204-337425 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References