Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3036143..3036762 Replicon chromosome
Accession NZ_CP082154
Organism Klebsiella pneumoniae strain Kp11-81

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6977_RS14665 Protein ID WP_002892050.1
Coordinates 3036143..3036361 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6977_RS14670 Protein ID WP_002892066.1
Coordinates 3036388..3036762 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6977_RS14630 (K6977_14620) 3032190..3032453 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
K6977_RS14635 (K6977_14625) 3032453..3032593 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6977_RS14640 (K6977_14630) 3032590..3033288 - 699 WP_002892021.1 GNAT family protein -
K6977_RS14645 (K6977_14635) 3033389..3034840 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6977_RS14650 (K6977_14640) 3034815..3035285 - 471 WP_002892026.1 YlaC family protein -
K6977_RS14655 (K6977_14645) 3035306..3035446 + 141 WP_004147370.1 hypothetical protein -
K6977_RS14660 (K6977_14650) 3035418..3035984 - 567 WP_002892030.1 maltose O-acetyltransferase -
K6977_RS14665 (K6977_14655) 3036143..3036361 - 219 WP_002892050.1 HHA domain-containing protein Toxin
K6977_RS14670 (K6977_14660) 3036388..3036762 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6977_RS14675 (K6977_14665) 3037248..3040394 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6977_RS14680 (K6977_14670) 3040417..3041610 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214238 WP_002892050.1 NZ_CP082154:c3036361-3036143 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T214238 NZ_CP110016:c3331565-3331251 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT214238 WP_002892066.1 NZ_CP082154:c3036762-3036388 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT214238 NZ_CP110016:c3331254-3330838 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References