Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1411078..1411724 Replicon chromosome
Accession NZ_CP082154
Organism Klebsiella pneumoniae strain Kp11-81

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag K6977_RS06955 Protein ID WP_002920560.1
Coordinates 1411377..1411724 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag K6977_RS06950 Protein ID WP_002920557.1
Coordinates 1411078..1411377 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6977_RS06930 (K6977_06920) 1407284..1408042 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
K6977_RS06935 (K6977_06925) 1408092..1408922 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
K6977_RS06940 (K6977_06930) 1408973..1409302 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
K6977_RS06945 (K6977_06935) 1409507..1411015 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
K6977_RS06950 (K6977_06940) 1411078..1411377 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
K6977_RS06955 (K6977_06945) 1411377..1411724 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K6977_RS06960 (K6977_06950) 1411900..1414347 - 2448 WP_002920561.1 glycogen phosphorylase -
K6977_RS06965 (K6977_06955) 1414365..1415798 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T214232 WP_002920560.1 NZ_CP082154:c1411724-1411377 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T214232 NZ_CP110016:c2178728-2178477 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT214232 WP_002920557.1 NZ_CP082154:c1411377-1411078 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT214232 NZ_CP110016:c2178480-2178226 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References