Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 962990..963644 Replicon chromosome
Accession NZ_CP082129
Organism Escherichia coli strain NDM_11.16372

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K5P77_RS04690 Protein ID WP_000244777.1
Coordinates 963237..963644 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K5P77_RS04685 Protein ID WP_000354046.1
Coordinates 962990..963256 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P77_RS04660 (958159) 958159..958902 + 744 WP_000951964.1 SDR family oxidoreductase -
K5P77_RS04665 (958959) 958959..960392 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
K5P77_RS04670 (960437) 960437..960748 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
K5P77_RS04675 (960912) 960912..961571 + 660 WP_000250274.1 hemolysin III family protein -
K5P77_RS04680 (961767) 961767..962747 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K5P77_RS04685 (962990) 962990..963256 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K5P77_RS04690 (963237) 963237..963644 + 408 WP_000244777.1 protein YgfX Toxin
K5P77_RS04695 (963684) 963684..964205 - 522 WP_001055874.1 flavodoxin FldB -
K5P77_RS04700 (964317) 964317..965213 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K5P77_RS04705 (965238) 965238..965948 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K5P77_RS04710 (965954) 965954..967687 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T214179 WP_000244777.1 NZ_CP082129:963237-963644 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214179 NZ_CP110014:c2178475-2178221 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214179 WP_000354046.1 NZ_CP082129:962990-963256 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214179 NZ_CP110014:c2178723-2178472 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References